Sharif Digital Repository

Separation of biomolecules such as enzymes is the most important part of biotechnology process which 10-90 % of costs is for separation and purification. There are several methods for purification of bio materials and one of them is chromatography which use in enzyme purification but this method have several problem such as cost ,time consuming and scale up Because of this a suitable alternative demands for industrial separation. An alternative is aqueous two-phase systems (ATPS). In this project was studied about partial purification of alkaline protease produced by Bacillus licheniformis ATCC 21424 in aqueous two-phase system and comparison with chromatography method. an aqueous two-phase... 

The present study focus is on finding a new solution to control laminar separation bubble phenomenon by combustion actuators in micro scale, which is investigated in flows with low Reynolds number surface effect and free flows. The main parameters in this problem are classified in three groups of formation location of laminar separation bubble, its length, and transition from laminar to turbulent around airfoil SD8020 in attack angle of 5 and 8 degree. Following that, the new combustion actuators in micro scale method the comparison it with cold (and hot) air jet injection is introduced to control boundary layer flow with separation bubble point of view. Some mechanisms are identified in...