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Study of a Biosensor Based on Nanofibers

Shamloo, Arezoo | 2011

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 41678 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vossoughi, Manouchehr; Alemzadeh, Iran
  7. Abstract:
  8. In this project an enzymatic peroxidase biosensor based on two naono structured material 1-Polyaniline nananofibers 2-Polyethylene glycol-Poly citric acid copolymer was studied for phenol detection.Polyaniline nanofibers and poly ethylene glycol-poly citric acid copolymer were synthetized by chemical polymerization.Electrochemical behaviors of these biosensors were studied using cyclic voltammetry (CV) and amperometric techniques. The amperometric response of the coated electrodes is highly stable with 92% of the initial activity remaining after 5 determinations. These phenol biosensors show an oxidation peak at 0.55V. The amperometric response for phenol biosensor based on Polyaniline showed a linear response range from2.5×10-6 molL-1 to 2.5×10-5 molL-1 , with a detection limit of 2.5 µM phenol and a correlation coefficient of 0.996 (n=11). Also The amperometric response for phenol biosensor based on poly ethylene poly citric acid showed a linear response range from2.5×10-6 molL-1 to 4×10-5 molL-1 , with a detection limit of 1.5 µM phenol and a correlation coefficient of 0.998 (n=15).Effects of other parameters (temperature, pH, H2O2 concentration,Applied potential) on these biosensors were studied too.Results show that both of nano structured materials are useful but polyethylene glycol-poly citric acid copolymer efficiency is much better.

  9. Keywords:
  10. Biosensor ; Phenol ; Horseradish Peroxidase (HPR) ; Polyaniline Nanofibers ; Polyethylene Glycol Polycitric Acid Copolymer

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