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Liquid-liquid-liquid microextraction and high performance liquid Chromatography for Seperation and Determination of Estrogens in Aquatic Media

Maleki, Hossein | 2011

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 42666 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Bagheri, Habib
  7. Abstract:
  8. Liquid-liquid-liquid microextraction (LLLME) is a new miniaturized liquid extraction to preconcentrate and purify for ionizable analytes. In LLLME firstly analytes extracted in an organic phase, and subsequently back-extracted into a second aqueous phase. Main advantages of LLME are their speed, simplicity, negligible volume of solvents, analytical precision, short sample preparation and low cost. This technique was shown to be an efficient preconcentration method for the extraction of estrone and estriol from biological samples. A basic microdrop in the tip of microsyringe was fixed in the organic layer over the sample solution. After extraction for a preset time, the microdrop was retracted back into the syringe and injected directly into an injection loop of HPLC. Effect of various parameters such as nature of organic solvent, volume of organic solvent, temperature, microdrop volume, ionic strength, pH, time of extraction from donor phase to organic phase, time of back-extraction and volume of organic phase and microdrop were investigated and optimized. The analytical data exhibited an enrichment factor of 80 for estriol and 111 for estrone, dynamic linear range of 1-500 µg L-1 and limit of detection of 3 µg L-1
  9. Keywords:
  10. High Performance Liquid Chromatography ; Estrogens ; Estrone ; Estriol ; Liquid Three-Phase Microextraction

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