Cell Separation via Nano-porous Molecularly Imprinted Polymer

Rahnema Falavarjani, Eiman | 2012

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 44027 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Abdekhodaie, Mohammad Jafar; Nematollah Zade, Ali
  7. Abstract:
  8. Molecularly imprinted polymers (MIPs) with synthetic recognition sites are capable of detecting and separating chemical species in complex environments. These characteristics are of increasing interest for bioanalytical applications. The chief advantages of MIPs are mechanical and chemical stability, low cost, and ease of preparation. Recently, imprinting technology has been used for fabrication polymer thin films with recognition sites of proteins, plant viruses, and yeasts. Also few investigations applied lithography for synthesis of cell imprinted polymers targeting cell detection and observation of cell morphology.
    For the first time, we demonstrate surface imprinting for the recognition of stem cells and cancer cells. Unlike lithography (Stamp like), living detached (suspended) cells were used for implementation of cell impression on the polymers, it leads increase of cell recognition. To accomplish a successful human cell imprinting, the living cells’ sites, not the dead cells’ ones, have to be fabricated on the polymer. Therefore, the cell imprinting process (includes Polymerization in presence of cell, Cell removal and, Cell Rebinding) must be fully biocompatible. Regarding to this fact the in-vitro biological system was developed with regulation of cell viability and pH value.
    After adjustment of biological system during cell imprinting, the imprinted polymer thin films were synthesized according novel cell imprinting method with name of “Upward Cell Imprinting”. Consequently, Mesenchymal stem cells separation in single type cell solution achieved by recognition sites on surface imprinted polymer thin films, the results surprisingly showed the 80 % occupying of sites with entering cells during few minutes.
    Also separation of the human osteosarcoma cells ( MG-63 ) in single and multiple( in presence of two other cancer cell lines: breast cancer (MCF-7) and human colonic (HT-25)) cells solution successfully achieved. This accomplishment will be clinically applicable as cancer cells detection and also, as new treatment of cancer by elimination of cancer cells from patient
  9. Keywords:
  10. Separation ; Alginate ; Stem Cell ; Cell Imprinting ; Molecularly Imprinted Polymers

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