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Design and Construction of PCL Nanofiber Scaffold for Bladder Tissue Engineering

Vakilian, Saeed | 2012

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 44284 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Yaghmaei, Soheyla; Mashayekhan, Shohreh
  7. Abstract:
  8. In this project, a novel system of bioactive electrospun scaffold for bladder tissue engineering , has been investigated to control in vitro cell differentiation, and utilize in in vivo vascularization and tissue formation. This method doesn’t have custom bioactive scaffolds problems such as, protein instability, technical complexity and, difficulties in accurate kinetics prediction. First of all, protein loaded chitosan nanoparticles based on ionic gelation interaction between chitosan and Sodium tripolyphosphate were prepared. Maximum protein loading efficiency (80% for BSA & 99% for TGF-beta1) in chitosan nanoparticles was obtained at mean diameter of 51nm. Moreover polycaprolactone electrospun fibers containing protein loaded chitosan nanoparticles, with parallel arrangement and mean diameter of 2 micrometer were prepared. In order to obtain desired mechanical properties and, tissue regeneration with enough elasticity for expansion and contraction, we made scaffolds biomimetic to bladder tissue by use of PCL/NP-PLLA hybrid nanofibers. This scaffold showed a fiber diameter ratio similar to the collagen-elastin fiber ratio in bladder extracellular matrix. A novel multi-layered structure of nanofibers synthesized in our research, was used to get a sustained release of the protein. The MTT assay showed, Gelatin coated PCL-PLLA, Gelatin coated Mono.PCL/NP-PLLA and, Gelatin coated Multi.PCL/NP-PLLA nanofiber scaffolds have enough adherence sites for Mesenchymal stem cell adhesion, migration and proliferation. Finally, bioassay of TGF-β1 released from scaffold, indicated the toxic solvents and the high voltage electric field have not effect on the growth factor bioactivity
  9. Keywords:
  10. Tissue Engineering ; Nanoparticles ; Nanofiber ; Bioactivity ; Bladder ; Growth Factor

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