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Fabrication and Characterization of a Drug Release System Based on Mesoporous Silica Nanoparticles for Hydrophobic Drugs

Taebnia, Nayyera | 2013

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 45619 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Yaghmaei, Soheila; Arpanaei, Ayyoob; Morshedi, Dina
  7. Abstract:
  8. This research aims to develop a drug delivery system based on mesoporous silica nanoparticles (MSNPs) for hydrophobic drugs and evaluating their cytotoxicity. The internal environment of the body is aqueous, while most of effective drugs display poor aqueous solubility, resulting in insufficient bioavailability. Due to their several unique properties, such as a large surface area, tunable pore size, facile surface multi functionalization and excellent biocompatibility, MSNPs are recognized as promising and powerful tools to overcome this hurdle. In the present study, MSNPs were synthesized using template removing method and then were functionalized through grafting procedure. They were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) isotherms, Zeta potential measurement and Fourier Transform Infrared Spectroscopy (FT-IR). Then isotherms of drug loading and its release from MSNPs were investigated for a model hydrophobic drug (Curcumin). At the last step, the effect of drug loaded nanoparticles on α-synuclein fibrillation and cytotoxicity was examined by ThT and MTT assay on PC12 cell line, respectively.
    SEM and TEM images revealed spherical structures with diameter of 83±6 nm. The N2 adsorption–desorption isotherms showed a type IV isotherm with 4.095 nm of pore size and 882.11 m2/g of surface area. Approximately 33% (w/w) of the curcumin was loaded on the Amine-functionalized MSNPs (A-MSNP) and was efficiently released into the aqueous phase. The results of MTT assay showed viability of more than 85% on PC12 cells and ThT results revealed up to 44% prevention of fibril formation.
  9. Keywords:
  10. Cytoxicity ; Mesoporous Silica Nanoparticles ; Drug Release ; Loading ; Hydropholic Drug ; Protein Fibrillation

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