Loading...

In Situ Synthesis of Nanorod Hydroxyapatite Reinforced Polycaprolactone Nanocomposite for Bone Tissue Engineering Application

Moeini, Saeed | 2014

813 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 45825 (07)
  4. University: Sharif University of Technology
  5. Department: Materials Science and Engineering
  6. Advisor(s): Mohammadi, Mohammad Reza; Simchi, Abdol Reza
  7. Abstract:
  8. Hydroxyapatite is the most substantial inorganic constituent of bone tissue which displays splendid biocompability and bioactivity. Nevertheless, its mechanical properties is not utmost appropriate for a bone substitutes. Therefore, it is used to improve the mechanical properties of polymer matrix composite scaffolds. In the present work polycaprolactone as a polymeric matrix was employed to fabricate hydroxyapatite-polycaprolactone biocomposite scaffolds via in situ route. Solvothermal method was employed to synthesize in situ hydroxyapatite in polymer matrix. Porous scaffolds were fabricated via freeze-drying/porogen leaching. Physical, mechanical (compressive module and compressive strength), biocampability and bioactivity of nanocomposites and scaffolds were studied by different analysis such as XRD and FTIR. It was observed that hydroxyapatite nanorods with diameter and aspect ratio in the range of 30-70nm and4-12 were obtained, respectively.Bioactivity of scaffolds was studied by immersing them in simulated body fluid for 1, 2 and 4 weeks. MTT assay were conducted in 48 and 72 hours to evaluate the biocampability of the scaffolds. MTT results showed that thein-situ fabricated scaffolds had higher Cell Proliferation than ex-situ prepared scaffolds. The nanocomposite containing with 20wt% hydroxyapatite had compressive modulus of and compressive strength in 10% strain of 241.6MPa and 19MPa,respectively. These parameters were improved 50% and 26%,respectively in comparison tomonolithic polycapralactone.Compressive module of 1.99MPa was observed for scaffold composed of 20wt% hydroxyapatite which showed33% increment compared to the scaffolds fabricated by ex-situ method
  9. Keywords:
  10. Bone Tissue Engineering ; Nanocomposite ; Hydroxyapatite ; Nanorod ; Polycaprolactone Composite

 Digital Object List

 Bookmark

No TOC