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Axial potential mapping of optical tweezers for biopolymer stretching: The bead size matters

Ahmadi, A ; Sharif University of Technology | 2013

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  1. Type of Document: Article
  2. DOI: 10.1364/OL.38.000685
  3. Publisher: 2013
  4. Abstract:
  5. Optical tweezers (OT) are widely used for pico (and femto)-Newton range force measurements. The appropriate choice of the bead size is not well understood for biopolymer stretching applications of OT. We have shown, both by theory and experiment, that wrong choice of the bead size could cause errors as large as 295% in the measured force. We provide a simple map for correct choice of the bead size and the direction of pulling for such applications. There is a good agreement between our theoretical and experimental results
  6. Keywords:
  7. Bead size ; Potential mapping ; Theoretical and experimental ; Biomolecules ; Optical tweezers ; Biopolymer ; Microsphere ; Chemistry ; Particle size ; pliability ; Biopolymers ; Microspheres ; Pliability
  8. Source: Optics Letters ; Volume 38, Issue 5 , 2013 , Pages 685-687 ; 01469592 (ISSN)
  9. URL: https://www.osapublishing.org/ol/abstract.cfm?uri=ol-38-5-685