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Experimental Study of Biocompatibility and Proliferation of Stem Cells on the Scaffold with Natural Structure

Zamani, Masoud | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 47592 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vosoughi, Manouchehr; Alemzadeh, Iran; Haghighipour, Nooshin
  7. Abstract:
  8. The heart diseases and in particular coronary artery disease have been vastly increased in recent years. Bypass operation is the major recognized way of this disease treatment however, one third of patients don’t have the suitable autologous vein for this operation. It should be noted that for this operation a suitable conduit with diameter as narrow as possible is needed. On the other hand, usage of synthetic polymers won’t be suitable for this operation due to the coagulation forming. The only way to overcome this problem is the use of tissue engineering with the aid of scaffold and autologous cells with the natural tissue function, which reconstructed the desire tissue causing no inflammation and sensitivity. In this work, the tubular composite scaffold was built based on natural silk protein. In order to fabricate the aforementioned scaffold, a tubular mesh of silk yarn was created to reinforce the structure and then a porous wall with appropriate pore distribution was achieved by freeze drying method. In the next step, to eliminate the formation of blood clot within the scaffold, heparin was covalently linked to the lumen surface of the scaffold. This biocompatible scaffold showed an appropriate mechanical properties. According to the experimental results, the scaffold has compliance, burst pressure and suture retention force equal to 3.9, 3500 mmHg and 1.2N, respectively which is comparable with those of saphenous vein. Also hemocompatibility assay showed that the scaffold made in this work has the anticoagulant activity for at least 28 days, which is due to the presence of heparin. It is anticipated that the development of this new construction will open new horizons towards coronary artery desease treatment
  9. Keywords:
  10. Stem Cell ; Biocompatibility ; Composite Structures ; Scaffold ; Silk Fibroin

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