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Fabrication of porous gelatin-chitosan microcarriers and modeling of process parameters via the RSM method

Karimian, S. A. M ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijbiomac.2016.03.061
  3. Publisher: Elsevier B.V
  4. Abstract:
  5. Porous gelatin-chitosan microcarriers (MCs) with the size of 350 ± 50 μm were fabricated with blends of different gelatin/chitosan (G/C) weight ratio using an electrospraying technique. Response surface methodology (RSM) was used to study the quantitative influence of process parameters, including blend ratio, voltage, and syringe pump flow rate, on MCs diameter and density. In the following, MCs of the same diameter and different G/C weight ratio (1, 2, and 3) were fabricated and their porosity and biocompatibility were investigated via SEM images and MTT assay, respectively. The results showed that mesenchymal stem cells (MSCs) could attach, proliferate, and spread on fabricated porous MCs during 7 days of culturing especially on those prepared with a G/C weight ratio of 1. Such porous gelatin-chitosan MCs with a G/C weight ratio of 1 may be considered as a promising candidate for injectable carriers supporting attachment and proliferation of MSCs
  6. Keywords:
  7. Cell expansion ; Gelatin/chitosan ; Microcarriers ; Response surface methodology ; Gelatin chitosan microcarrier ; Nanoparticle ; Unclassified drug ; Chitosan ; Drug carrier ; Gelatin ; Article ; Biocompatibility ; Bend ratio ; Cell adhesion ; Cell culture ; Cell spreading ; Chemical parameters ; Chemical structure ; Electric potential ; Eectrospray ; Flow rate ; Human ; Human cell ; Mechanical stability ; Mechanics ; Microcarrier density ; Microcarrier diameter ; MTT assay ; Process model ; Response surface method ; Scanning electron microscopy ; Surface tension ; Syringe pump flow rate ; Chemistry ; Cytology ; Drug effects ; Electrochemical analysis ; Factor analysis ; Female ; Mesenchymal stroma cell ; Cell Proliferation ; Cell Survival ; Electrochemical Techniques ; Factor Analysis, Statistical ; Mesenchymal Stromal Cells ; Porosity ; Pregnancy ; Primary Cell Culture ; Tissue Scaffolds ; Umbilical Cord
  8. Source: International Journal of Biological Macromolecules ; Volume 88 , 2016 , Pages 288-295 ; 01418130 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0141813016302975