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Preparation and Evaluation of Doxorubicin-loaded Fe3O4\Chitosan Magnetic Nanocomposite for Drug Delivery System in Cancer Therapy

Tajeri, Razieh | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 49865 (07)
  4. University: Sharif University of Technology
  5. Department: Materials Science and Engineering
  6. Advisor(s): Maddah Hosseini, Hamid Reza
  7. Abstract:
  8. Recent drug delivery strategies have attempted to maximize the concentration of chemotherapeutic molecules into the tumors, while minimizing their systemic distribution. Doxorubicin has been widely used for a variety of cancers, successfully producing regression in acute leukaemia, lymphomas, soft-tissue and osteogenic sarcomas, paediatric malignancies and adult solid tumours, in particular breast and lung carcinomas. Common adverse effects of doxorubicin include hair loss, myelosuppression, nausea and vomiting, oral mucositis, oesophagitis, diarrhoea, skin reactions and serious reactions include hypersensitivity reactions, radiation recall, heart damage and liver dysfunction. Chitosan has prompted the continuous impetus for the development of safe and effective drug delivery systems because of its unique physicochemical and biological characteristics. The primary hydroxyl and amine groups located on the backbone of chitosan allow for chemical modification to control its physical properties. Superparamagnetic iron oxide nanoparticles (SPIONs) are attractive materials that have been widely used in medicine for drug delivery, diagnostic imaging, and therapeutic applications. This Magnetically responsive NPs must possess properties such as biocompatibility, absence of toxicity and immunogenicity, appropriate drug vehiculization capabilities and significant responsiveness to the magnetic gradients. The approach of this research relies on nanoplatforms that can experience a change in their physicochemical properties under exposure to an external stimulus (acidic pH, magnetic gradients), leading to a specific concentration of the drug into the tumor interstitium. In this study SPIONs and the anticancer drug, doxorubicin hydrochloride, were encapsulated into chitosan coating by the reverse microemulsion and suspension crosslinking technique. Hyperthermia and chemotherapy and MRI could be accomplished simultaneously with Doxorubicin-loaded Fe3O4\chitosan magnetic nanocomposite designed in this project. this research describe reproducible technique for the preparation of magnetic core/shell NPs consisting of a SPIONs nucleus and a chitosan shell, and loaded with the anticancer drug Doxorubicin. A common problem encountered during doxorubicin–chitosan encapsulation can be attributed to the same cationic, hydrophilic nature of doxorubicin and chitosan. Doxorubicin-loaded Fe3O4\chitosan magnetic nanocomposite characterization has been used via VSM, FT-IR, DLS, UV-Vis, TGA. The silanization process and the coating efficiency of chitosan around the magnetic core has been analyzed using FTIR. The TGA results confirmed the amount of chitosan coating on the surface of oxide nanoparticles. The amount of doxorubicin loaded to the core/shell NPs has been investigated by UV-Vis spectrophotometry. The magnetic properties of the nanocomposites were evaluated by VSM to analyze NPs magnetic responsiveness
  9. Keywords:
  10. Drug Delivery ; Chitosan ; Doxorubicin ; Superparamagnetic Nanoparticles ; Superparamagnetic Iron Oxide Nanoparticle ; Targeted Druy Delivery ; Cancer Treatment ; PH-triggered Release

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