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Trace determination of free formaldehyde in DTP and DT vaccines and diphtheria-tetanus antigen by single drop microextraction and gas chromatography-mass spectrometry

Bagheri, H ; Sharif University of Technology | 2009

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  1. Type of Document: Article
  2. DOI: 10.1016/j.jpba.2009.04.039
  3. Publisher: 2009
  4. Abstract:
  5. An immersed single drop microextraction (SDME) method was successfully developed for the trace enrichment of formaldehyde from DTP and DT vaccines and diphtheria-tetanus antigen. The formaldehyde was derivatized by means of the Hantzsch reaction. The dehydropyridine derivative was extracted into a microdrop of chloroform that suspended in a 4 ml sample solution for a preset time. The microdrop was then retracted into the microsyringe and injected directly into a gas chromatography-mass spectrometry (GC-MS) injection port. Effects of different parameters such as the type of solvent, extraction time, stirring rate, and temperature were studied and optimized. The limit of detection was 0.22 ng/l and relative standard deviation (RSD) value was 6.2% (n = 5). The regression coefficient was satisfactory (r2 = 0.992) and linear range was obtained from 1 to 500 ng/l. © 2009 Elsevier B.V. All rights reserved
  6. Keywords:
  7. DT vaccines and diphtheria-tetanus antigen ; DTP ; Formaldehyde ; Immersed single drop microextraction ; Antigen ; Chloroform ; Diphtheria pertussis tetanus vaccine ; Diphtheria tetanus antigen ; Diphtheria tetanus vaccine ; Formaldehyde ; Uunclassified drug ; Article ; Chemical reaction ; Drug determination ; Ddrug isolation ; Mass fragmentography ; Ppriority journal ; Single drop microextraction ; Solvent extraction ; Antigens, Bacterial ; Diphtheria ; Diphtheria-Tetanus Vaccine ; Diphtheria-Tetanus-Pertussis Vaccine ; Formaldehyde ; Gas Chromatography-Mass Spectrometry ; Solvents ; Tetanus
  8. Source: Journal of Pharmaceutical and Biomedical Analysis ; Volume 50, Issue 3 , 2009 , Pages 287-292 ; 07317085 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S073170850900257X