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Investigation on Conductivity and Piezoelectric Effects on Biological Properties of Conductive Nerve Regenerating Scaffolds by Nano Composite of Gelatin/Polyvinyl Fluoride/Graphene

Sahrayi, Hamid Reza | 2018

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 51914 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Ramazani Saadat Abadi, Ahmad; Bakhshandeh Abkenar, Haleh; Atyabi, Mohammad
  7. Abstract:
  8. Neural system in human body due to limitated cell proliferation requires high care, because the damage causes serious hurts and disables different tissues. Limit regeneration in central neural system can cause different neural diseases including brain exhaustions specially in old people, but the peripheral neural system may have been disabled and intrupt some other tissue beacuase of possible external injuries before aging. So that while some damages like neural fibers severing, some new medical care methods are required. Some of these methods are outograft, allograft and xenograft which implants neural tissues either between other patient's tissues or from somebody else's; however these methods have some critical limitations like causing tumor and postponing human body immune system. So that neural tissue engineering is one of the most productive ways in such situations; thus the project follows some investigations through neural tissue engineering, which include fabrication and evaluation of biocompatible and biodegradable scaffolds. To fulfil this purpose the scaffolds have been fabricated by biocompatible Gelatin and Ployvinylidene fluoride. Polyaniline has been used to improve electrical conductivity of the scaffolds. In this project the porous scaffolds have been fabricated by electrospinning and they have been characterized by physical and biological tests. Analysis used in this project include final tests to approve synthesis polymers and also the scaffold fabrication methods and required charactrizations. Scaffold biocompatibality has been analysed and approved by invitro tests using two different cell line, PC12 and C6. Also electrical stimulation has been used in different durations to evaluate proliferation of seeded cells on scaffolds which has been analysed using MTT and SEM tests. In conclusion, electrospun Gelatin/PVDF scaffods containing Polyaniline could be a reliable alternative implant for peripheral nerve injuries
  9. Keywords:
  10. Tissue Engineering ; Conductive Polymer ; Nervous System ; Electrical Conductivity ; Gelatin ; Polyvinylidene Fluoride (PVDF) ; Nerve Tissue Engineering

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