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Fabrication of a Collagen-Based Scaffold for Corneal Regeneration

Alizadeh, Maryam | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52335 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Abdekhodaie, Mohammad Jafar
  7. Abstract:
  8. Corneal diseases are one of the most common causes of blindness worldwide. The most common way to treat these diseases is to replace the damaged cornea with the donor cornea by corneal transplant. But limitations such as donor tissue deficiency, the risk of disease transmission and transplant rejection have made treatment of these diseases very difficult. A cornea made by tissue engineering can be used as a suitable alternative because of the limitations mentioned for corneal transplantation with donated tissue. At the same time, similarity of the scaffold to normal corneal tissue, both in terms of matrial and structural properties, is essential for proper repair of this scaffold by cells after replacement with damaged tissue. For this purpose, in this study, a collagen scaffold consisting of fibers and hydrogels similar to the normal structure of the cornea was made of collagen, the major constituent biomolecule of the corneal stroma to repair and replace corneal stroma tissue. For this purpose, collagen was extracted from rat tail and the extracted collagen was charechterised by SDS-PAGE and CD tests. Then, collagen orthogonal fibers with curved structure similar to normal corneal tissue were fabricated by electrospinning method. The collagen hydrogel was also fabricated using EDC/NHS crosslinking. After optimization, the final scaffold was fabricated by incorporating collagen fibers and hydrogels and its performance was evaluated by SEM, water absorption, degradation, suture permeability and transparency. The results showed that the scaffold designed in this study not only possesses structural properties similar to normal cornea, but also has the strength required for suturing in corneal transplantation and can be used as a scaffold for repair of corneal tissue
  9. Keywords:
  10. Cornea ; Collagen ; Electrospinning ; Hydrogel ; 1-Ethyl-3-(3-dimethylaminopropyl)Carbodiimide ; Corneal Tissue Regeneraction

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