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Fabrication, modeling and optimization of lyophilized advanced platelet rich fibrin in combination with collagen-chitosan as a guided bone regeneration membrane

Ansarizadeh, M ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijbiomac.2018.12.078
  3. Publisher: Elsevier B.V , 2019
  4. Abstract:
  5. In this study, lyophilized advanced platelet rich fibrin (A-PRF) was used in combination with collagen-chitosan membrane for the first time to combine advantages of both collagen and A-PRF membranes. Response surface methodology (RSM) was used to design the experimental condition and to correlate the effects of parameters, including chitosan/collagen (chit/col) weight ratio and A-PRF concentration on Young's modulus, mesenchymal stem cell (MSCs) viability and degradation rate of the membranes. Results showed that Young's modulus of the membranes was intensified by increasing chit/col weight ratio and decreasing A-PRF concentration from 3 to 8 MPa. Cell viability of MSCs was improved by both increasing chit/col weight ratio and A-PRF concentration. Moreover, as chit/col weight ratio increased from 0 to 4 and A-PRF concentration decreased from 5 to 0, degradation rate of the membranes decreased from 90 to 20% after four weeks incubation. Finally, based on Design Expert Software calculation for minimizing the degradation rate and maximizing both Young's modulus and cell viability, the values of chit/col weight ratio and A-PRF concentration were suggested to be 4 and 0.58 mg/ml, respectively. Alkaline phosphatase (ALP) activity analysis showed that the addition of A-PRF caused higher osteogenic differentiation
  6. Keywords:
  7. Advanced platelet rich fibrin ; Collagen membrane ; Response surface methodology ; Alkaline phosphatase ; Chitosan nanoparticle ; Fibrin ; Lyophilized advanced platelet rich fibrin ; Unclassified drug ; Biological marker ; Platelet-rich fibrin ; Animal cell ; Cell differentiation ; Cell membrane ; Cell viability ; Chitosan collagen weight ratio ; Controlled study ; Degradation ; Enzyme activity ; Fourier transform infrared spectroscopy ; Incubation time ; Mechanics ; Mesenchymal stem cell ; Mouse ; Nanofabrication ; Nonhuman ; oOsteoblast ; Physical parameters ; Process optimization ; Response surface method ; Scanning electron microscopy ; Software ; Young modulus ; Animal ; Artificial membrane ; Cytology ; Human ; Metabolism ; Procedures ; Spectroscopy ; Tissue regeneration ; Animals ; Biomarkers ; Bone Regeneration ; Cell Survival ; Chitosan ; Collagen ; Freeze Drying ; Guided Tissue Regeneration ; Humans ; Membranes, Artificial ; Mesenchymal Stem Cells ; Mice ; Spectrum Analysis
  8. Source: International Journal of Biological Macromolecules ; Volume 125 , 2019 , Pages 383-391 ; 01418130 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0141813018348918