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Design, Fabrication and Evaluation of Polymeric Microcapsules Containing Cells to Use in Cell Therapy in Heart

Hosseini, Mahshid | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54095 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Sharifi, Ali Mohammad; Khanmohammadi, Mehdi
  7. Abstract:
  8. Because of the inability of conventional methods to regenerate the infarcted part of the heart, regenerative medicine based on using scaffolds and hydrogels incorporation with cells and biological factors emerges as a promising approach. Micro-structures, especially hollow microcapsules created by the microfluidic system, has received a great deal of attention due to their inspired biomimetic structures, providing complete coverage cells, and preventing an immune response, consequently. This study aimed to use a microfluidic system to fabricate hollow microcapsules and investigate the behavior of rat cardiomyoblast cells (H9C2) along with exosomes within these structures. These structures' membrane was a combination of two synthetic polymers, alginate-phenol, and gelatin-phenol, polymerized by enzymatic reactions. Synthetic polymers were evaluated by spectrophotometer, gelation time, and Proton nuclear magnetic resonance assays. After optimizing the microfluidic system to produce hollow microcapsules with different diameters in the range of 90 to 194 μm, swelling, strength, and mechanical stability assays were performed. Mechanical tests' results indicated 84% and 100% stability of hollow microcapsules against stresses caused by horizontal shaker and osmotic pressure, making them suitable for use in the infarcted myocardium. The cardiomyoblast model cells' behavior was investigated inside hollow microcapsules with a diameter of 92 μm. MTT test and cell staining showed partially low cell growth and high cell viability after 21 days. In addition, peaks related to the size distribution of exosomes isolated under normal and hypoxia conditions were 109 and 154 nm using dynamic light scattering
  9. Keywords:
  10. Exosomes ; Microcapsules ; Microfluidic System ; Myocardial Infarction ; Cell Therapy ; Hollow Microcapsules

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