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Investigation and Characterization of Circulating tumor Cells (CTCs) by the Microfluidics Systems

Yari, Alireza | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54263 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vossoughi, Manoucher; Alemzadeh, Iran; Shamloo, Amir
  7. Abstract:
  8. Cancer is the second leading cause of death among humans as one of the major human concerns. More than 500,000 people die of cancer each year in the United States alone. Cancer mortality is mainly due to cancer metastasis. Metastasis is the escape of cancer cells from a primary tumor, the circulation of cells in the circulatory system (CTSs), the penetration of/from vessel wall, and the formation of a secondary tumor. It is still not clear how the cells survive in the circulatory system, along with the mechanical pressures and stresses that exist in the body's capillary channals. In this study, a microcirculation model for circulating cells with barriers was designed and constructed by the microfluidic system. The required flow for the device was calculated by simulation. MCF7 and MDA-MB-231 cancer cells entered to the microchannel and their behavior and movement were visualized and analyzed. The deformability of cells was investigated as a mechanical property. It was found that cells with higher metastatic properties, such as MDA-MB-231, had a shorter transit time than the MCF7 class, and could cross barriers easier than those have less metastasis properties. It was also found that the use of mechanical properties in the characterization of cells can be used as a criterion in the differentiation and identification of cancer cells
  9. Keywords:
  10. Characterization ; Microfluidic System ; Cell Mechanics ; Mechanical Properties ; Deformable Model ; Circulating Tumor Cells (CTC) ; Cancer Cells

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