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A Gold Nanoparticle-based Colorimetric Sensor Array for Detection and Discrimination of Antibiotics in Groundwater

Hassannia, Maryam | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54691 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Hormozinezhad, Reza
  7. Abstract:
  8. Antibiotics (ABs) have been regarded as powerful medications to treat or prevent bacterial infections. The antibiotic resistance though, is one of daunting challenges of our time which has been attributed to the overuse of these drugs and usage of antibiotic-contaminated food resources. Developing rapid and visual detection methods capable of simultaneously monitoring Abs is highly desired due to their harmful effects on human health. In the present study, we have designed a colorimetric sensor array consisting of two types of gold nanoparticles (i.e., Citrate-capped gold nanoparticles (Cit-AuNPs) and Borohydride-capped gold nanoparticles (BH4-AuNPs) for the discrimination and determination of critical ABs belong to different antibiotic classes (i.e., azithromycin (AZT), amoxicillin (AMOX), Ciprofloxacin (CIP), clindamycin (CLN), cefixime (CPX), Doxycycline (DOX) and metronidazole (MET)). The design principle of the probe was based on the aggregation of Cit-AuNPs and BH4-AuNPs in the presence of ABs. Unique aggregation profiles of sensor elements in the presence of the targets at various pH conditions, using citrate and phosphate buffers, were visually assessed and statistically analyzed by various data visualization and pattern recognition methods. Namely, linear discriminant analysis (LDA) and partial least squares (PLS) regression were employed for the qualitative and quantitative determination of ABs. The responses were linearly correlated to the concentrations of ABs in a wide range of 0.10-2.00, 0.05-2.00, 0.10-2.00,0.10-2.00, 0.10-0.50, 0.17-3.00, and 0.20-3.00 μg.mL–1 with the limit of detections of 0.05, 0.03, 0.04, 0.01, 0.06, 0.05 and 0.04 μg.mL–1 for AZT, AMOX, CIP, CLN, CFX, DOX, and MET, respectively. Finally, the practical applicability of the sensor array was investigated by the determination of BAs in river and underground water by which the potential of the probe for on-site determination of antibiotics was successfully verified
  9. Keywords:
  10. Colorimetric Sensor Array ; Antibiotic ; Gold Nanoparticle ; Pattern Recognition ; Ciprofloxacin Antibiotic ; Linear Discriminant Analysis ; Metronidasole ; Azithromycin ; Amoxicillin

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