Loading...

Investigation on Insulin Release from Microcapsules Containing Beta Cell

Hosseinzadeh, Zahra | 2022

187 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54988 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Alemzadeh, Iran; Vossoughi, Manouchehr
  7. Abstract:
  8. Type 1 diabetes, formerly known as insulin-dependent diabetes, may present at any age, but is the most common type of diabetes affecting children and adolescents. People with type 1 diabetes do not produce enough insulin. This type accounts for 5-10% of all types of diabetes. In this type of diabetes, beta cell destruction occurs in the pancreas. In type 1 diabetes, the pancreas does not secrete any insulin; insulin is a hormone that enables the body to convert glucose in food into energy. If sugar in the blood cannot enter the cells, it accumulates in the arteries and results in high blood sugar. High blood sugar over time can have irreversible effects on various parts of the body. Curcumin is a drug derived from turmeric that has been shown to have anti-inflammatory properties based on studies. The aim of this study was to encapsulate RIN-5F cells in alginate microcapsules made by extrusion method which have high water absorption, swelling and stability. RIN-5F cells were encapsulated with curcumin to induce anti-inflammatory properties and prevent microcapsules from being rejected by the immune system. The microcapsule was made with a diameter of 200-250 µm and had about 12 times the water absorption capacity. According to the results, there was no significant difference in the amount of insulin released and the survival rate of cells in three different states on days 1, 3 and 7. The lowest cell survival rate was observed on the first day which was about 81% and on the seventh day it reached the highest value of about 90%. With increasing cell viability, the amount of insulin secreted increased from about 30.3 µIU/ml on the first day to 191.29 µIU/ml on the seventh day, which was the highest amount recorded during seven days. As a result, curcumin has no negative effect on insulin secretion and cell survival, so it can be used as an anti-inflammatory for capsule transplantation. Also, the release rate of curcumin from microcapsules reached 0.26% in 24 hours, which indicates the long shelf life of curcumin in microcapsules
  9. Keywords:
  10. Microcapsules ; Beta Cell ; Encapsulation ; Alginate ; Curcumin ; Insulin Release ; Extended Release System

 Digital Object List

 Bookmark

No TOC