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Fullerene C60 nanoparticle attenuates pain and tumor necrosis factor-α protein expression in the hippocampus following diabetic neuropathy in rats

Namdar, F ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.52547/phypha.26.4.5
  3. Publisher: Iranian Society of Physiology and Pharmacology , 2022
  4. Abstract:
  5. Introduction: Diabetic neuropathy is a common complication of diabetes mellitus. It is associated with nerve damage due to oxidative stress and high levels of pro-inflammatory mediators. In the present study, we examined the anti-nociceptive effects of Fullerene nanoparticle, as a potent anti-oxidant, during diabetic neuropathy. Methods: Diabetes mellitus induced through injection of streptozotocin (STZ) (40 mg/kg). Four groups were used in the study as follows: the control, control+fullerene, diabetes, and diabetes +fullerene groups. All four groups received sesame oil. Treatment rats received fullerene C60 (1mg/kg/day) for 9 weeks by intra-gastric gavage. Then, cold allodynia, histology, and tumor necrosis factor-α (TNF-α) protein expression of the hippocampus were measured 9 weeks after injection of STZ. Results: Our data revealed that STZ induces cold allodynia in both hind paws and increases the TNF-α protein expression in the hippocampus. Furthermore, STZ induces neural degeneration in the hippocampus. Additionally, fullerene C60 significantly attenuated cold allodynia and TNF-α protein expression. Also, fullerene C60 has neuro-protective effects on hippocampal neurons. However, fullerene C60 did not significantly reduce serum glucose levels in diabetic animals. Conclusion: Our data suggest that fullerene C60 likely suppressed pain, and neural loss by inhibitory effects on TNF-α protein expression in the hippocampus during diabetes. © 2022, Iranian Society of Physiology and Pharmacology. All rights reserved
  6. Keywords:
  7. Fullerene C60 ; Hippocampus ; TNF-α ; Antioxidant ; Fullerene ; Nanoparticle ; Sesame seed oil ; Streptozocin ; Tumor necrosis factor ; Adult ; Animal experiment ; Animal model ; Animal tissue ; Cold allodynia ; Controlled study ; Densitometry ; Diabetic neuropathy ; Enteric feeding ; Glucose blood level ; Hind paw ; Histology ; Male ; Nerve cell ; Nerve degeneration ; Nonhuman ; Pain ; Protein expression ; Protein function ; Rat ; Single drug dose ; Western blotting
  8. Source: Physiology and Pharmacology (Iran) ; Volume 26, Issue 4 , 2022 , Pages 451-458 ; 24765236 (ISSN)
  9. URL: https://ppj.phypha.ir/article-1-1754-en.html