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Optimization of Lipase Production Process in Suitable Strain Culture

Esmaeili, Razieh | 2023

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 57032 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Askaripour, Hossein; Roostaazad, Reza; Yeganeh Sarkandi, Shahin
  7. Abstract:
  8. In this research, after examining the different strains available in the enzyme center's cell bank, the bacterial strain Pseudomonas aeruginosa (RPMC-B-3556) obtained from the Russian microbial collection was used for lipase production. This microbial strain has the ability to produce extracellular lipase enzyme and for this reason it has been selected as the target strain. The purpose of this research was to optimize the lipase production process in the culture of the appropriate strain. For this purpose, the compounds used in the culture medium are changed to investigate their effect on lipase enzyme activity. Considering that the studied strain has the ability to produce protease enzyme and the presence of protease affects the activity of lipase enzyme, in addition to measuring the activity of lipase enzyme, the activity of protease enzyme is also investigated. From the middle of this research, the percentage of inoculum was considered to be 1% according to the results of reference [1]. Also, pH = 8 was adjusted for the medium of the cultures and then these cultures were incubated at a temperature of 35 ºC and a rotation speed of 171 rpm. The results of this research showed that the highest activity of lipase enzyme is obtained in the basic culture medium without the presence of olive oil. In this research, the separation of two enzymes, lipase and protease, was investigated using a membrane, and the results showed that it is not possible to separate the two enzymes using a membrane, but passing the enzyme solution through the membrane increases the concentration of the enzyme, which as a result, the enzyme activity increases
  9. Keywords:
  10. Lipase Enzyme ; Alkaline Protease Enzyme ; Pseudomonas Aeruginosa Bacteria ; Culture Optimization ; Optimization

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