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Development of Structures based on Chitosan-Quince Seed Gum Polyelectrolyte Complex and Mesoporous Silica Nanoparticles for Drug Delivery Application

Kaviani, Alireza | 2024

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  1. Type of Document: Ph.D. Dissertation
  2. Language: Farsi
  3. Document No: 57364 (07)
  4. University: Sharif University of Technology
  5. Department: Materials Science and Engineering
  6. Advisor(s): Pircheraghi, Gholamreza; Bagheri, Reza
  7. Abstract:
  8. Polyelectrolyte complexes based on polysaccharides have attracted the attention of researchers due to their potential for use in advanced industries. One of the most widely used cationic polysaccharides is chitosan. However, chitosan alone limits applications for smart and sustainable drug delivery due to uncontrolled degradation. For this reason, combining chitosan with another polyelectrolyte and forming a polyelectrolyte complex due to electrostatic interaction has been proposed as a solution. In the first phase of this dissertation, the effects of mixing ratio and concentration of Chitosan and Quince seed gum (QSG) on the physical and rheological properties of chitosan-QSG polyelectrolyte complex were investigated. The oscillatory shear rheological behavior of the complexes showed that an elastic gel was formed. The critical entanglement concentration (Ce) for QSG was observed at 0.3% w/v. The complex viscosity of the complexes had shear-thinning behavior. The results of structural characterization confirmed that, in addition to hydrogen bonding, this complex was formed through electrostatic interaction between the amine groups of chitosan and the carboxyl groups of the glucuronic acid chains in QSG. The dye absorption test proved that this complex is able to absorb ionic dyes with different charges. The adsorption capacity and removal efficiency of cationic methylene blue (MB) was higher than Methyl Orange and Rhodamine B. Using a quartz crystal microbalance with dissipation (QCM-D) and ellipsometry, it was observed that the combination of chitosan and QSG has the ability to form multilayer polyelectrolyte films with dimensions below 100 nm. Voigt, Sauerbery and ellipsometry models calculated thickness as 107.8, 34.8 and 54.1 nm for 5 bilayers, respectively. It is demonstrate that Viscoelastic model estimated more accurately both the thickness and mass per unit area of constructed film due to the capacity of being highly swelled. The thickness of the multilayer films showed dependency on pH and salt concentration in the initial solutions as well as pH after construction. QCM-D analysis showed the process of drug absorption, swelling and release in a period of 3 hours. Film with 5 bilayers could absorb 1200 ng/cm2 Ibuprofen within the first hour. Ibuprofen drug release kinetics was investigated at two pH values of 2 and 7.4 and it was observed that there was a significant difference in the drug release mechanism so that the release at pH 2 was performed at a slower rate and follows the Korsmeier-Peppas model. In this research, mesoporous silica nanoparticles were successfully synthesized from biocompatible and natural Tannic acid template with an average diameter of 54.1 nm and a BET surface area of 321.3 m2/g. Sterile gauze samples were made by impregnation with mesoporous silica nanoparticles and chitosan-QSG polymeric coating based on structural and morphological analyzes in order to make an smart wound dressing. The release of Ciprofloxacin drug from nanoparticles showed that the kinetics of the drug depends on the number of layers and by changing the number of layers, the kinetics can be controlled. The dual release of anti-inflammatory drugs, Ibuprofen and Ciprofloxacin, were released from polymer coating and nanoparticles in a stable manner for 48 hours. The formulation developed in this dissertation has demonstrated its versatility as polyelectrolyte multilayer films and antibacterial wound dressings with sustained drug release capability
  9. Keywords:
  10. Polyelectrolyte ; Chitosan ; Quince Seed Gum ; Mesoporous Silica Nanoparticles ; Drug Delivery ; Silica ; Polyelectrolyte Complex

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