Sharif Digital Repository

Nowadays with the extension of industry and factories as well as different kinds of plants, a wide range of pollution and circumference hazardous has been entered to the people`s life. Industrial wastewater has been always a threat for health of our environment and efforts for reducing its dangerous effects are still under studies. Dairy wastewater is one of the main branches of food industrial pollutions and it has been studied in this specific project. The goal was to reduce the Chemical Oxygen Demands (COD) of dairy wastewater with oxidation of Titanium Dioxide Nanophotocatalyst oxidation under UV irradiation. Among the various methods for synthesizing of this Nanophotocatalyst, the... 

In this research, Response Surface Methodology was applied to study the effects of Na-Montmorillonite and glycerol on some functional properties of corn starch films. Films were prepared by casting method according to central composite design. No antimicrobial activities were observed against Escherichia coli and Staphylococcus aureus. The minimum of hydrophilicity and the maximum of tensile strength were obtained by maximization of clay and minimization of plasticizer in the considered range, which resulted in minimum transparency. Biodegradation of corn starch/glycerol/Na-MMT nanocomposites by soil burial according to ASTM D6400 and enzymatic degradation tests were carried out for up to 6... 

Sustainable development requires the development and promotion of environmental management and a constant search for green technologies to treat a wide range of aquatic and terrestrial habitats contaminated by increasing anthropogenic activities. In this work we investigated the ability of four previously isolated fungi, BBRC 9057, BBRC 20020, BBRC 9058 and BBRC 9002, in degradation of polycyclic (PAHs) and the effect of UV irritation on fungi cell growth and degradation of PAHs. Also we studied the degradation of anthracene by thermophilic filamentous fungi in liquid and soil slurry bioreactor. Cell growth and anthracene degradation ability of BBRC 9057 increased 6.51 and 1.82 times rather... 

In this study, differentiation of rabbit embryonic-derived mesenchymal stem cells to osteogenic cells has been characterized. Bone tissue engineering is based upon the understanding of bone tissue construct and it’s formation in-vivo, and the Preparation of tissue engineered bone constructs to repair large size defects is it’s major goal. We sought to investigate the combined effect of three elemnts of tissue engineering: cells, scaffolds and growth factors. Mesenchymal stem cells are unspecialized cells which due to their unlimited self-renewal capacity and the remarkable ability to differentiate along multiple linage pathways are natural choice for application in tissue repair and... 

L-asparaginase enzyme is a well known enzyme due to itschemotherapeutic properties. However, this is not the only feature of the enzyme. L- asparaginase can be also used in food processing to reduce of acrylamide which is a carcinogenic substance. furthermore, acrylamide formation, is prevented by addition of the enzyme before cooking process of some foods such as chips, which contains sugar and asparagine amino acid. Due to use of the enzyme in food industry, we need to produce enzyme with high activity and cheaper raw material. In the present study, Candida utilis ATCC 9950 was used to produce asparaginase enzyme, and beet molasses and yeast extract was used as a carbohydrate and... 

Vitamin C is an essential for the survival of human, animals and plants. Human and most animals can not produce vitamin C in their bodies, thus the need to produce vitamin C as a supplement is necessary. Nowadays, for the industrial production of vitamin C, two Chinese and Rychstyn methods are used, the first method is a two-step method which is accomplished biological and the second method is done chemically. In Chinese method, first step, which is the most important step is production of L-sorbose from D-sorbitol by Gluconobacter oxydans bacteria .Thus, in this study it was attempted to optimize the production of L-sorbose to be used in industrial production of L-ascorbic acid. In... 

Nowadays, Food generation has gained great importance, not only in economic view but also in political. Food reservation is as important as food generation. Synthetic polymer films has widespread use in packaging industries, that is because of its features like easily forming, low price, lightness, etc. The increase of oil resources prices and severe environmental pollution by synthetic polymers, are notable cases which led researchers to find ways in using bio polymers. Starch is one of the bio polymers which is cheap and totally degradable. Using starch in bio degradable leads to high decrease in the mix features. Nano particles are used to solve the problem.
In this study, the effect... 

Conjugated linoleic acid (CLA) is a collective term used to describe the mixture of geometric isomers of linoleic acid which has several beneficial biological effects. Considering the use of CLA for medicinal of nutraceutical purposes, an isomer-selective and safe process is required. The introduction of biological reactions to CLA production will solve this problems. Nowadays, CLA is produced through chemical isomerization of linoleic acid which results in by-production of unexpected isomers. From this point of view biotechnology methods and producing microorganisms implementation have gained lots of attention because of not producing unwanted bypass products and also being natural method.... 

Burns are one of the most important accidents related to human health. Due to the intense physical and mental complications and high fatality rate associated with them, receiving proper treatment is of paramount importance. The control of infection in wounds would cure and eliminates the effect of wounds and treatment of skin lesions with engineered scaffolds can be an effective method. The purpose of this project is proposing a hydrogel scaffold based on natural polymers of oxidized alginate and gelatin loaded with an herbal drug to control infection and treat burn wounds. For this purpose, the Iranian Oak extract that it's main content is Tanin and PolyPhenolinc materials, was prepared and... 

Cancer is a major cause of mortality worldwide, with a disease burden estimated to grow over the coming decades. Circulating tumor cells (CTCs) are rare cancer cells released from the primary or metastatic tumors and transported though the peripheral circulatory system to their specific secondary locations. The presence of CTCs in a cancer patient’s blood has been used as a prognostic biomarker, with lower CTC count correlating with greater overall survival. In spite of its clinical potential, the isolation and detection of CTCs has been a challenging task due to its rare presence amongst other blood cells (as low as 1–10 CTCs per billions of blood cells) and variability in terms of both... 

This study aimed to prepare a type of microcapsule that microencapsulates folic acid and iron at the same time and protects these two substances against environmental and gastrointestinal conditions. Simultaneous release of these two nutrients has dual health properties and improves the efficiency of folic acid microencapsulation. In this study, biocomposites were formed containing alginate, pectin, and carboxymethylcellulose, and the microcapsules were synthesized by the ionization of calcium-alginate ions. Biocomposite optimization mixture with 13 experiments using experimental design software, optimal combination design method based on repopulation separator, restoring folic acid... 

Combined composites consisting of nanofibers and hydrogels were fabricated to grow cells in an environment that mimicked the extracellular matrix. Electrospan nanofibers were spun and hydrolyzed under optimized conditions consisting of polycaprolactone (PCL) and gelatin and then incorporated into alginate and gelatin hydrogels. Nanofibril-containing (NF / hydrogel) hydrogel hybrids were ligated by calcium chloride in the presence of encapsulated beta cells. The mechanical properties of NF / hydrogel scaffolds were significantly increased in proportion to the NF content in NF / hydrogels. Beta cells killed in NF / hydrogels showed higher adhesion to the matrix compared to samples without NF.... 

Convenient medical diagnostics and treatments can replace conventional healthcare procedures because of their fast and profitable applications with reduced infection risk. Meanwhile, early diagnosis of cancer is leading research subject due to the high fatality rate of the disease and being a vital precursor in the treatment effectiveness. Electrochemical-based micro-biosensors are the best candidates for measuring biomarker concentrations in body fluids. To address this need, we used a simple fabrication approach to develop an effective immunosensor for the early detection of cancer, in particular prostate cancer. The carbon fibers, obtained from the electrospinning technique, decorate the... 

Application of nanoparticles in targeted delivery and controlled drug release is recently highly attracted especially in cancer therapy. In this study with the aim of developing a novel drug vector based on metallic nanoparticles, gold nanoparticles with their unique characteristics were used to design a vector for targeted anti-cancer drug delivery to tumor. First, gold nanoparticles were chemically synthesized therough sodium citrate and sodium brohydrate reductions with average diameters of 22 and 11 nm, respectively. Then, the activated poly ethylene glycol (PEG) and activated folic acid were conjugated to the lysine-capped gold nanoparticles. Subsequently, the widely used anticancer... 

Peppermint oil is one of the most often used food ingredients and it is volatile. Many common procedures such as microencapsulation have been used for protecting volatile food ingredients. For this research, L-menthol was chosen as the core material to be encapsulated. L-menthol is responsible for the minty flavor and cooling sensation of food and pharmaceutical products. The encapsulation of L-menthol was prepared by complex coacervation, using gum Arabic and gelatin as wall material and tannic acid as cross-linking agent. The effect of various processing parameters, including wall material, core material, tannic acid and tween¬80 concentrations, on particle size and efficiency was... 

L-Asparaginase has two major uses. For decades it has been known and used as an antitumor agent. Besides, it has been introduced as a food processing aid in the last trench against acrylamide formation in foods. Here, we have produced extracellular L-Asparaginase from Candida utilis and studied the parameters surrounding its production. Thus, two species of yeast were cross-examined: Saccharomyces cerevisiae (BBRC 30006) and Candida utilis (ATCC 9950). Moreover, the most suitable method for detecting and determining L-Asparaginase activity was investigated and selected for this purpose which is via L-Aspartate determination with Hydroxylamine. Furthermore, six deferent parameters of... 

With the rapid development of biotechnology, peptide and protein drugs are now playing an increasingly important role in therapeutics. Compared with chemical drugs, peptide and protein drugs have some limitations such as low stability and rapid deactivation. Biodegradable and biocompatible polymeric micro carriers have been shown to have a high potential for the delivery of peptides and proteins. Among these polymers, alginate has been widely investigated as a biomaterial. Alginates are natural polysaccharide polymers isolated from brown seaweed. Bovine serum albumin (BSA) loaded calcium alginate microspheres produced in this study by a modified w/o emulsification method. The influence of... 

The dairy industry wastewater has high biological oxygen demand(BOD) and high chemical oxygen demand(COD). In this study, membrane bioreactor(MBR) was used for the treatment dairy industry wastewater. Since two diffuser,submerged membrane modul and intermittent suction method were adpted to system. In this study, the response surface method and experimental design were applied as an alternative to conventional methods for the optimization of the treatment. A central composite design, with 5 replicates at the center point were used to build a model for predicting and optimizing the treatment. Mathematical model equations were derived by computer simulation programming with Design Expert... 

Disposal of molasses wastewater of Alcohol production and sugar refinery plants into water resources make irreparable damages to the environment. Continuous disposal of this wastewater causes decreases soil quality and destroys agricultural products. Wastewater which is produced from molasses alcoholic fermentation causes numerous environmental issues due to the dark color and high level of organic compounds. Most of these organic compounds can be eliminated by aerobic or anaerobic biological treatments. Nevertheless, the color which is due to the presence of heavy organic compounds, named melanoidin, in wastewater is hardly removed by conventional biological treatments. In this project, the...