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    Experimental Study of Biological Synthesis, Stabilization and Delivery of the Recombinant Protein of Chondroitinase

    , Ph.D. Dissertation Sharif University of Technology Askaripour, Hossein (Author) ; Vossoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor) ; Khajeh, Khosro (Supervisor)
    In this research, immobilization method was employed to increase the thermal stability of Chondroitinase ABC I (cABC I) enzyme. In first step, magnetite nanoparticle (Fe3O4) was selected as a support and cABC I enzyme was attached via the adsorption method. The results showed that pH=6.5, temperature 15 ˚C, enzyme-to-support mass ratio 0.75, and incubation time 4.5 hr were the appropriate conditions for immobilizing cABC I enzyme on Fe3O4 nanoparticle. It was also found that the optimum pH for free and immobilized enzymes was 7.6 and 8.0, respectively. The maximum activity of free enzyme was obtained at 25 ˚C, whereas the activity of immobilized enzyme was almost constant in the temperature... 

    Investigation and Characterization of Circulating tumor Cells (CTCs) by the Microfluidics Systems

    , M.Sc. Thesis Sharif University of Technology Yari, Alireza (Author) ; Vossoughi, Manoucher (Supervisor) ; Alemzadeh, Iran (Supervisor) ; Shamloo, Amir (Supervisor)
    Cancer is the second leading cause of death among humans as one of the major human concerns. More than 500,000 people die of cancer each year in the United States alone. Cancer mortality is mainly due to cancer metastasis. Metastasis is the escape of cancer cells from a primary tumor, the circulation of cells in the circulatory system (CTSs), the penetration of/from vessel wall, and the formation of a secondary tumor. It is still not clear how the cells survive in the circulatory system, along with the mechanical pressures and stresses that exist in the body's capillary channals. In this study, a microcirculation model for circulating cells with barriers was designed and constructed by the... 

    Experimental Study of Circulating Tumor Cell (CTC) by Application of Microfluidic Systems and its Related Characterization Studies

    , Ph.D. Dissertation Sharif University of Technology Hakim, Maziar (Author) ; Khorasheh, Farhad (Supervisor) ; Vossoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    Isolation and characterization of circulating tumor cells (CTCs) found in blood samples of cancer patients have been considered as a reliable source for cancer prognosis and diagnosis. A new continuous microfluidic platform has been designed in this investigation for simultaneous capture and characterization of CTCs based on their deformability. The deformability-based chip (D-Chip) consists of two sections of separation and characterization where slanted weirs with a gap of 7 μm were considered. Although sometimes CTCs and leukocytes have the same size, the deformability differs in such a way that can be exploited for enrichment purposes. MCF7 and MDA-MB-231 cell lines were used for the... 

    Investigation of Microbial Electrolysis Cell Using Wastewater from Food Industries

    , M.Sc. Thesis Sharif University of Technology Jafari Chamgordani, Behnaz (Author) ; Alemzadeh, Iran (Supervisor) ; Kariminia, Hamid Reza (Supervisor)
    Using microbial electrolysis cells (MEC) for hydrogen production has been considered in recent years. So far, synthetic wastewaters containing substances such as glucose and acetate have been mainly used in MECs and industrial wastewaters have not been widely studied. Food industry wastewater is a suitable choice for application in microbial electrolysis cell due to its biodegradable materials. In present research, dairy wastewater and sludge collected from UASB reactor of Iran Dairy Industries company, Tehran were initially used in a dual chamber microbial fuel cell until a stable voltage was generated .Afterward, the system was converted to a MEC applying a predetermined voltage. The... 

    Experimental Study of Biocompatibility and Proliferation of Stem Cells on the Scaffold with Natural Structure

    , M.Sc. Thesis Sharif University of Technology Zamani, Masoud (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor) ; Haghighipour, Nooshin (Supervisor)
    The heart diseases and in particular coronary artery disease have been vastly increased in recent years. Bypass operation is the major recognized way of this disease treatment however, one third of patients don’t have the suitable autologous vein for this operation. It should be noted that for this operation a suitable conduit with diameter as narrow as possible is needed. On the other hand, usage of synthetic polymers won’t be suitable for this operation due to the coagulation forming. The only way to overcome this problem is the use of tissue engineering with the aid of scaffold and autologous cells with the natural tissue function, which reconstructed the desire tissue causing no... 

    Study and Fabrication of a Multilayer Scaffold Containing Biological Agents for Skin Wounds Regeneration

    , Ph.D. Dissertation Sharif University of Technology Hajiabbas, Maryam (Author) ; Alemzadeh, Iran (Supervisor) ; Vossoughi, Manouchehr (Supervisor) ; Shamloo, Amir (Co-Supervisor)
    In recent years, it is expected that the fabrication of multilayer scaffolds and the use of different methodologies in one product can be a new progressing method in skin substitute production. Accordingly, this project aims to fabricate a bilayered composite scaffold with a combination of hydrogel and electrospinning method. We have tried to prepare a scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) without using corrosive solvents and toxic crosslinking agents as a scaffold and drug delivery system. As different biological, chemical, physical, and mechanical factors play a vital role in the healing process, we have characterized the proposed scaffold via DSC,... 

    Synthesis of Metal-organic Frameworks (MOFs) Nano-hybrids for Removal of Organic Pollutants from Petrochemical and Colored Wastewaters

    , Ph.D. Dissertation Sharif University of Technology Abdi, Jafar (Author) ; Vossoughi, Manouchehr (Supervisor) ; Mahmoodi, Niyaz Mohammad (Supervisor) ; Alemzadeh, Iran (Supervisor)
    Physical processes such as adsorption and membrane filtration are in progress and considered as efficient methods in separation and treatment of organic pollutants from petrochemical and colored wastewaters. The advantages of these methods include high energy efficiency, low cost, facile process, and regeneration and implementation ability for a wide range of contaminants. Nevertheless,requirement of suitable materials as adsorbents with high adsorption capacity, capability of preparing membranes based on these materials, optimization of process conditions and removal improvement have still remained as the main challenges; therefore, these topics have been mentioned as main aims of this... 

    Study, Optimization and Construction of a Microfluidic Gene Amplification Device by Using Thin Film Layer Method

    , M.Sc. Thesis Sharif University of Technology Eslami, Sara Sadat (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Co-Supervisor) ; Shamloo, Amir (Co-Supervisor)
    Polymerase Chain Reaction (PCR) is a process in which a special piece of a gene is amplified millions of times over a short period. This method has been of paramount importance in different fields of research and has been applied for different applications. PCR requires thermal cycling, or repeated temperature changes between two or three discrete temperatures to amplify specific nucleic acid target sequences. To achieve such thermal cycling, conventional bench-top thermal cyclers generally use a metal heating block powered by Peltier elements or benefit from forced convection heat transfer. Due to the fact that these methods are time consuming, it seems that design and fabrication of a fast... 

    Comparative Examination and Preparation of Polymeric Hydrogels in Order to use as Cellular Scaffold

    , M.Sc. Thesis Sharif University of Technology Nazaripouya, Amir (Author) ; Alemzadeh, Iran (Supervisor) ; Sharifi Aghdas, Farzane (Supervisor) ; Mashayekhan, Shohre (Supervisor)

    Study & Synthesis of Biocompatible Scaffolds for Animal Cell Cultures

    , M.Sc. Thesis Sharif University of Technology Riazi Esfahani, Bahar (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Co-Advisor) ; Pajoum Shariati, Ramin (Co-Advisor)
    Natural tissues of body consist of Extra Cellular Matrix (ECM) and cells. Scaffold produces a temporary matrix for cells and facilitates oxygen and nutrients transport which enhances tissue regeneration. Therefore, attempts in regeneration of tissues and organs by scaffolds attracted great interest in today’s researchs. Choosing scaffold fabrication method and its polymer type, play a key role in scaffold specifications as they determine scaffold properties. In this project electrospinning was chosen as the scaffold fabrication method which is because of the unique properties of scaffolds fabricated by this method and also its ability in producing micro and nano fibers. At first set of... 

    Design and Fabrication of Biodegradable Polymeric Scaffold with nano-Bioglass for Osteoblast cell Growth

    , M.Sc. Thesis Sharif University of Technology Razaghzadeh Bidgoli, Mina (Author) ; Vossoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor) ; Tamjid Shabesteri, Elnaz (Co-Advisor)
    Treatment of critical-size bone defects caused by sport injuries, accidents, trauma, infection, and osteoporosis remains a major clinical challenge. In order to repair or regenerate large bone defects, bioactive three-dimensional scaffolds play a key role due to their multilevel porous structure, high surface area, enhanced mass transport and diffusion. Many studies reported that macropore diameters greater than 500 µm can lead to vascularized bone tissue. In this study, a hierarchically porous composite scaffold was prepared. Hierarchically porous silk fibroin- bioactive glass composite and fibroin scaffold were fabricated with controlled architecture and interconnected structure with... 

    Design, Expression, and Evaluation of Nanoparticle Structure Containing Malaria Vaccine Candidate Antigen by Self-assembled Peptide Nanoparticles (SAPNs) and Confirmation of its Antigenicity Using Serum of Malaria Patients

    , M.Sc. Thesis Sharif University of Technology Zahedi, Farhad (Author) ; Alemzadeh, Iran (Supervisor) ; Abouei Mehrizi, Akram (Supervisor) ; Sardari, Soroush (Co-Supervisor) ; Zakeri, Sedigheh (Co-Supervisor)
    Malaria is one of the most important infectious diseases in the tropical and subtropical regions of the world and is caused in humans by the bite of the female Anopheles mosquito of five species of Plasmodium. According to the World Health Organization, about 229 million people worldwide were infected in 2019 by the disease, and 409,000 people died from the disease. Resistance of the parasite against drugs and resistance of vectors to insecticides and larvicides, which prevents the elimination and eradication of malaria, shows the need for new strategies, such as having an effective vaccine against this disease. In the second generation of vaccines, to develop malaria vaccines, recombinant... 

    A study on plant polymer: pectin production and modification

    , Article International Journal of Engineering, Transactions B: Applications ; Volume 23, Issue 2 , 2010 , Pages 107-114 ; 1728-144X (ISSN) Alemzadeh, I ; Sharif University of Technology
    Pectin from two different plant sources: apple and sugar beet was studied. Apple pectin was modified by chemical treatments such as: acid, alkali and ammonia, so as to obtain an ester level equal to or less than 50 %, which is described as low methoxyl (LM) pectin. LM pectin form gels with significant characteristic. In this study, de-esterification of apple pectin with acid treatment resulted in gel specification with higher molecular weight and viscosity. Acid treatment resulted in pectin with average molecular weight of 32000 Dalton. Pectin was extracted from sugar beet pulp by acid and alkali treatments. The quality of gel formation and water uptake was improved using sugar source... 

    The study on microbial polymers: Pullulan and PHB

    , Article Iranian Journal of Chemistry and Chemical Engineering ; Volume 28, Issue 1 , 2009 , Pages 13-21 ; 10219986 (ISSN) Iran, A ; Sharif University of Technology
    Microbial cells are producers of natural polymers present in plant cells. Production of pullulan (an extracellular microbial polysaccharide) by Aureobasidium pullularia pullulans (P. pullulans) was studied under fermentation conditions, and kinetic parameters were determined. Pullulan formation obeyed a growth and non-growth associated term. PHB (polyhydroxybutyrates) an intracellular biopolymer production by Rastonia eutropha (Alcaligen eutrophus), R. eutropha was studied under different culture media, including synthetic and natural carbon sources. Molasses as a natural carbon source in the culture media presented high efficiency in cell and biopolymer accumulation  

    Bio alcohol production from agricultural residues

    , Article 3rd International Symposium on Biotechniques for Air Pollution Control, Delft, 28 September 2009 through 30 September 2009 ; 2010 , Pages 167-174 ; 9780415582704 (ISBN) Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
    The bioconversion of abundant and renewable cellulosic biomass into ethanol as an alternative to petroleum is gaining importance due to the realization of diminishing natural oil and gas resources. Agricultural and foresty plant residues are an abundant and renewable source of sugar substrates that could be fermented to ethanol. A thermochemical treatment of biomass in which both cellulose and hemicellulose are hydrolyzed to soluble sugar is necessary before yeast fermentation. After thermochemical treatment, cellulase enzymes must be introduced in the system to hydrolyze any remaining cellulose. The simultaneous saccharification and fermentation (SSF), is a method which converts... 

    Phenols removal by immobilized horseradish peroxidase

    , Article Journal of Hazardous Materials ; Volume 166, Issue 2-3 , 2009 , Pages 1082-1086 ; 03043894 (ISSN) Alemzadeh, I ; Nejati, S ; Sharif University of Technology
    Application of immobilized horseradish peroxidase (HRP) in porous calcium alginate (ca-alginate) for the purpose of phenol removal is reported. The optimal conditions for immobilization of HRP in ca-alginate were identified. Gelation (encapsulation) was optimized at 1.0% (w/v) sodium alginate in the presence of 5.5% (w/v) of calcium chloride. Upon immobilization, pH profile of enzyme activity changes as it shows higher value at basic and acidic solution. Increasing initial phenol concentration results in a decrease in % conversion. The highest conversion belongs to phenol concentration of 2 mM. Investigation into time course of phenol removal for both encapsulated and free enzymes showed... 

    Removal of phenols with encapsulated horseradish peroxidase in calcium alginate

    , Article Iranian Journal of Chemistry and Chemical Engineering ; Volume 28, Issue 2 , 2009 , Pages 43-49 ; 10219986 (ISSN) Alemzadeh, I ; Nejati, S ; Sharif University of Technology
    Horseradish peroxidase was encapsulated in calcium alginate for the purpose of phenol removal. Considering enzyme encapsulation efficiency, retention activity and enzyme leakage of the capsules, the best gelation condition was found to be 1 % w/v of sodium alginate solution and 5.5 % w/v of calcium chloride hexahydrate. Upon immobilization, pH profile of enzyme activity changes as it shows higher value at basic and acidic solution. Besides, for each phenol concentration there would be an enzyme concentration which going further than this value has no significant effect on phenol removal. Investigation into time course of phenol removal for both encapsulated and free enzyme showed that... 

    Controlled release of paraquat from poly vinyl alcohol hydrogel

    , Article Chemical Engineering and Processing ; Volume 41, Issue 8 , 2002 , Pages 707-710 ; 02552701 (ISSN) Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
    A thorough investigation on different controlled release systems were evaluated. The required devices were constructed simultaneously and the required experiments were performed. The polymeric network and membranes of poly vinyl alcohol with cross-linking agent glutaraldehyde for preparation of hyrogel system was effected. Paraquat, a type of herbicide was introduced as solute in the system. Glutaraldehyde was utilized as cross-linking agent at different concentration. Increase in the concentration of the cross-linking agent will cause a decrease in the extent of release out of the system. The effect of temperature on hydrogel adsorption demonstrates a higher adsorption at lower temperature.... 

    Date syrup and baker's yeast production

    , Article Industrial and Engineering Chemistry Research ; Volume 41, Issue 1 , 2002 , Pages 128-130 ; 08885885 (ISSN) Alemzadeh, I ; Vosoughi, M ; Sharif University of Technology
    American Chemical Society  2002
    Date syrup might be considered as one of the innovative and attractive byproducts in date processing for the habitants in west and southwest Asia. Date syrup, which is an important byproduct of dates, was obtained by water extraction and evaluated as a substrate for baker's yeast production, in a batch laboratory-scale fermentation. Saccharomyces cerevisiae was cultivated in the date syrup (20 g/L) based medium. Batch growth was studied in shaken flasks. The diauxic growth was observed with two specific growth rates, 0.22 and 0.035 h-1, where the first relates to date sugar consumption and the second to ethanol uptake. Addition of a corn steep liquor (CSL) increased the dry cell mass, and a... 

    Oil biodegradation in sea water

    , Article Iranian Journal of Science and Technology, Transaction B: Technology ; Volume 25, Issue 1 , 2001 , Pages 87-92 ; 03601307 (ISSN) Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
    Biodegradation of petroleum hydrocarbons in salt medium (SM) containing sea water by Pseudomanas sp. was investigated. Different oil hydrocarbons such as n-decane, benzene, naphthalene, paraffin, paraffin + vaseline, paraffin + kerosene, and fuel oil were used as carbon sources. Cell growth (OD) and COD were measured for evaluation of oil biodegradability. The effect of inorganic and organic nitrogen sources on COD removal revealed a 95% and 75% reduction of COD for inorganic and organic nitrogen sources, respectively