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    Do the soft tissues located outside tibiofemoral joint have a role in bearing the compressive loads of the joint? An in-vitro study on sheep stifle joints

    , Article Gazzetta Medica Italiana Archivio per le Scienze Mediche ; Volume 172, Issue 7-8 , 2013 , Pages 595-601 ; 03933660 (ISSN) Hakkak, F ; Rostami, M ; Parnianpour, M ; Jabalameli, M ; Sharif University of Technology
    Aim. The compressive loads on the tibiofemoral joint are normally assumed to be borne solely via contact and pressing of the cartilage surfaces of tibia and femur. However, recent findings suggest that non-contact load-bearing mechanisms are active in the joint as well. In this context, a non-contact load-bearing mechanism involving soft tissue connections outside the tibiofemoral joint has been hypothesized as well. This paper addresses the validity of this hypothesis and the possible involvement of several soft tissue connections outside the joint. Methods. Sheep stifle (knee) joints were studied in vitro. The specimens were loaded in fixed displacement. Various soft tissues outside the... 

    Effect of mozart music on hippocampal content of BDNF in postnatal rats

    , Article Basic and Clinical Neuroscience ; Volume 2, Issue 3 , 2011 , Pages 21-26 ; 2008126X (ISSN) Marzban, M ; Shahbazi, A ; Tondar, M ; Soleimani, M ; Bakhshayesh, M ; Moshkforoush, A ; Sadati, M ; Zendehrood, S. A ; Joghataei, M. T ; Sharif University of Technology
    Introduction: It has shown that listening to Mozart music can potentiate spatial tasks in human; and reduce seizure attacks in epileptic patients. A few studies have reported the effects of prenatal plus postpartum exposure of mice to the Mozart music on brain-drived neurotrophic factor (BDNF) in the hippocampus. Here we investigated the effect of postpartum exposure to The Mozart music on BDNF concentration in the hippocampus of rat. Methods: Thirty male one day old newborn Wistar rats divided randomly in two equal experimental and control groups. Experimental group exposed to slow rhythm Mozart music (Mozart Sonata for two pianos KV 448, 6 hour per day; sound pressure levels, between 80... 

    A simple and efficient total synthesis of (±)-danshexinkun A, a bioactive diterpenoid from Salvia miltiorrhiza

    , Article Tetrahedron Letters ; Volume 51, Issue 3 , 2010 , Pages 540-542 ; 00404039 (ISSN) Matloubi Moghaddam, F ; Moridi Farimani, M ; Sharif University of Technology
    An efficient 12-step route for the synthesis of the diterpenoid quinone (±)-danshexinkun A in 23% overall yield from the corresponding highly substituted stilbene using a photocyclization strategy is described  

    SAR thresholds for electromagnetic exposure using functional thermal dose limits

    , Article International Journal of Hyperthermia ; Volume 34, Issue 8 , 2018 , Pages 1248-1254 ; 02656736 (ISSN) Adibzadeh, F ; Paulides, M. M ; Van Rhoon, G. C ; Sharif University of Technology
    Taylor and Francis Ltd  2018
    Background and purpose: To protect against any potential adverse effects to human health from localised exposure to radio frequency (100 kHz–3 GHz) electromagnetic fields (RF EMF), international health organisations have defined basic restrictions on specific absorption rate (SAR) in tissues. These exposure restrictions incorporate safety factors which are generally conservative so that exposures that exceed the basic restrictions are not necessarily harmful. The magnitude of safety margin for various exposure scenarios is unknown. This shortcoming becomes more critical for medical applications where the safety guidelines are required to be relaxed. The purpose of this study was to quantify... 

    Evaluation of the effects of fullerene c60 nanoparticles on oxidative stress parameters in normal rats liver and brain

    , Article Journal of Advances in Medical and Biomedical Research ; Volume 27, Issue 124 , 2019 , Pages 8-15 ; 26766264 (ISSN) Namadr, F ; Bahrami, F ; Bahri, Z ; Ghanbari, B ; Elahi, S. A ; Mohammadi, M. T ; Sharif University of Technology
    Zanjan University of Medical Sciences and Health Services  2019
    Background & Objective: The potent antioxidant property of fullerene C60 nanoparticles and their derivatives has been demonstrated in a wide range of in vitro and in vivo studies. Hence, we examined the effects of fullerene C60 on the oxidative stress parameters in brain and liver of the rats in normal situation. Materials & Methods: The study was performed in two groups of Wistar rats (each group, n = 6); normal and fullerene-treated normal animals. Treated rats received fullerene via oral gavage at dose of 1 mg/kg/day for 60 days. At termination of the study, the oxidative stress parameters were determined in brain and liver tissues, including the contents of glutathione (GSH) and... 

    Comparison of strengths of five internal fixation methods used after bilateral sagittal split ramus osteotomy: An in vitro study

    , Article Dental Research Journal ; Volume 17, Issue 4 , 2020 , Pages 258-265 Sarkarat, F ; Ahmady, A ; Farahmand, F ; Fateh, A ; Kahali, R ; Nourani, A ; Rakhshan, V ; Sharif University of Technology
    Wolters Kluwer Medknow Publications  2020
    Results on the strength and displacement of internal fixation methods for bilateral sagittal split ramus osteotomy are controversial, and some designs have not been adequately studied. Therefore, this study was conducted to compare techniques using bicortical or monocortical screws. Materials and Methods: In this in vitro study, 35 sheep hemi-mandibles were randomly assigned to five groups of seven each: fixation using (1) a 13 × 2 screw, (2) two 13 × 2 screws (arranged vertically), (3) three 13 × 2 screws, (4) 1 plate with 4 holes and four monocortical screws, and (5) a Y-shaped plate and five monocortical screws. Specimens underwent vertical forces until failure. Breakage forces and... 

    The influence of temperature alterations on eccentric contraction-induced isometric force and desmin loss in ratmedial gastrocnemius muscle

    , Article Journal of Medical Sciences ; Volume 8, Issue 2 , 2008 , Pages 162-169 ; 16824474 (ISSN) Vasaghi Gharamaleki, B ; Keshavarz, M ; Gharibzadeh, S ; Sotodeh, M ; Marvi, H ; Mosayebnejad, J ; Ebrahimi Takamjani, I ; Sharif University of Technology
    In this study isolated perfused rat muscle was used to examine the direct effect of temperature changes on the eccentric contraction-induced force and desmin loss. The left medial gastrocnemius muscle was separated and the entire lower limb was transferred into a prewarmed (35°C) organ bath. Temperature was adjusted to 31 or 39°C before and during eccentric contractions. Maximal isometric force and desmin loss were measured after 15 isometric or eccentric contractions. According to our data, organ bath temperature changes before or during eccentric contractions had no significant effect on force loss. However, a strong correlation between desmin loss and temperature changes before (r = 0.93,... 

    Simulation of blood oxygenation in capillary membrane oxygenators using modified sulfite solution

    , Article Biophysical Chemistry ; Vol. 195, issue , Dec , 2014 , p. 8-15 Tabesh, H ; Amoabediny, G ; Rasouli, A ; Ramedani, A ; Poorkhalil, A ; Kashefi, A ; Mottaghy, K ; Sharif University of Technology
    Blood oxygenation is the main performance characteristic of capillary membrane oxygenators (CMOs). Handling of natural blood in in vitro investigations of CMOs is quite complex and time-consuming. Since the conventional blood analog fluids (e.g. water/glycerol) lack a substance with an affinity to capture oxygen comparable to hemoglobin's affinity, in this study a novel approach using modified sulfite solution is proposed to address this challenge. The solution comprises sodium sulfite as a component, simulating the role of hemoglobin in blood oxygenation. This approach is validated by OTR (oxygen transfer rate) measured using native porcine blood, in two types of commercially available... 

    Disc size markedly influences concentration profiles of intravenously administered solutes in the intervertebral disc: A computational study on glucosamine as a model solute

    , Article European Spine Journal ; Vol. 23, issue. 4 , April , 2014 , p. 715-723 Motaghinasab, S ; Shirazi-Adl, A ; Parnianpour, M ; Urban, J. P. G ; Sharif University of Technology
    Purpose: Tests on animals of different species with large differences in intervertebral disc size are commonly used to investigate the therapeutic efficacy of intravenously injected solutes in the disc. We hypothesize that disc size markedly affects outcome. Methods: Here, using a small non-metabolized molecule, glucosamine (GL) as a model solute, we calculate the influence of disc size on transport of GL into rat, rabbit, dog and human discs for 10 h post intravenous-injection. We used transient finite element models and considered an identical GL supply for all animals. Results: Huge effects of disc size on GL concentration profiles were found. Post-injection GL concentration in the rat... 

    Real-time simulation of the nonlinear visco-elastic deformations of soft tissues

    , Article International Journal of Computer Assisted Radiology and Surgery ; Volume 6, Issue 3 , 2011 , Pages 297-307 ; 18616410 (ISSN) Basafa, E ; Farahmand, F ; Sharif University of Technology
    Purpose: Mass-spring-damper (MSD) models are often used for real-time surgery simulation due to their fast response and fairly realistic deformation replication. An improved real time simulation model of soft tissue deformation due to a laparoscopic surgical indenter was developed and tested. Method: The mechanical realization of conventional MSD models was improved using nonlinear springs and nodal dampers, while their high computational efficiency was maintained using an adapted implicit integration algorithm. New practical algorithms for model parameter tuning, collision detection, and simulation were incorporated. Results: The model was able to replicate complex biological soft tissue... 

    A new model of pressure propagation in human tissue in HIFU application

    , Article Acta Medica Mediterranea ; Volume 31, Issue 7 , 2015 , Pages 1501-1505 ; 03936384 (ISSN) Hajian, S. R ; Abbaspour Tehrani Fard, A ; Pouladian, M ; Hemmasi, G. R ; Sharif University of Technology
    A. CARBONE Editore  2015
    This project is a new pressure model for propagating pressure inside one or several tissues at the time of treatment with high intensity focus ultrasound (HIFU). Pressure's changes are converted to heat's changes in tissue and this is done oscillatory. When the treatment is done in deeper tissues, obtained heat can affect surficial tissues. This pressure effect also can enter surficial tissues. Amount of pressure and heat possible injury can be controlled and reduced through this mechanical modelling. In this model we have used three layers and pressure also is obtained within these three layers and is investigated averagely. Obtained tissue in this mood is kidney tissue and it is tried to... 

    Developmental barcoding of whole mouse via homing CRISPR

    , Article Science ; Volume 361, Issue 6405 , 2018 ; 00368075 (ISSN) Kalhor, R ; Kalhor, K ; Mejia, L ; Leeper, K ; Graveline, A ; Mali, P ; Church, G. M ; Sharif University of Technology

    Construction of scaffolds composed of acellular cardiac extracellular matrix for myocardial tissue engineering

    , Article Biologicals ; Volume 53 , 2018 , Pages 10-18 ; 10451056 (ISSN) Esmaeili Pourfarhangi, K ; Mashayekhan, S ; Ghanbari Asl, S ; Hajebrahimi, Z ; Sharif University of Technology
    Academic Press  2018
    High rates of mortality and morbidity stemming from cardiovascular diseases unveil extreme limitations in current therapies despite enormous advances in medical and pharmaceutical sciences. Following myocardial infarction (MI), parts of myocardium undergo irreversible remodeling and is substituted by a scar tissue which eventually leads to heart failure (HF). To address this issue, cardiac patches have been utilized to initiate myocardial regeneration. In this study, a porous cardiac patch is fabricated using a mixture of decellularized myocardium extracellular matrix (ECM) and chitosan (CS). Results of rheological measurements, SEM, biodegradation test, and MTT assay showed that the... 

    Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model

    , Article Journal of Cellular Physiology ; Volume 234, Issue 11 , 2019 , Pages 20742-20754 ; 00219541 (ISSN) Mohammadshirazi, A ; Sadrosadat, H ; Jaberi, R ; Zareikheirabadi, M ; Mirsadeghi, S ; Naghdabadi, Z ; Ghaneezabadi, M ; Fardmanesh, M ; Baharvand, H ; Kiani, S ; Sharif University of Technology
    Wiley-Liss Inc  2019
    A large number of treatment approaches have been used for spinal cord injury improvement, a medically incurable disorder, and subsequently stem cell transplantation appears to be a promising strategy. The main objective of this study is to ascertain whether combinational therapy of human neural stem cells (hNSCs) together with lithium chloride improves cell survival, proliferation, and differentiation in a rat spinal contusion model, or not. Contusive spinal cord injury was implemented on Wistar male rats. Experimental groups comprised of: control, hNSCs transplanted, lithium chloride (Li), and hNSCs and lithium chloride (hNSCs + Li). In every experimental group, locomotor activity score and... 

    Temporal activation of LRH-1 and RAR-γ in human pluripotent stem cells induces a functional naïve-like state

    , Article EMBO Reports ; Volume 21, Issue 10 , 2020 Taei, A ; Kiani, T ; Taghizadeh, Z ; Moradi, S ; Samadian, A ; Mollamohammadi, S ; Sharifi Zarchi, A ; Guenther, S ; Akhlaghpour, A ; Asgari Abibeiglou, B ; Najar Asl, M ; Karamzadeh, R ; Khalooghi, K ; Braun, T ; Hassani, S. N ; Baharvand, H ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Naïve pluripotency can be established in human pluripotent stem cells (hPSCs) by manipulation of transcription factors, signaling pathways, or a combination thereof. However, differences exist in the molecular and functional properties of naïve hPSCs generated by different protocols, which include varying similarities with pre-implantation human embryos, differentiation potential, and maintenance of genomic integrity. We show here that short treatment with two chemical agonists (2a) of nuclear receptors, liver receptor homologue-1 (LRH-1) and retinoic acid receptor gamma (RAR-γ), along with 2i/LIF (2a2iL) induces naïve-like pluripotency in human cells during reprogramming of fibroblasts,... 

    Nonlinear mechanics of soft composites: hyperelastic characterization of white matter tissue components

    , Article Biomechanics and Modeling in Mechanobiology ; Volume 19, Issue 3 , 2020 , Pages 1143-1153 Yousefsani, S. A ; Shamloo, A ; Farahmand, F ; Sharif University of Technology
    Springer  2020
    This paper presents a bi-directional closed-form analytical solution, in the framework of nonlinear soft composites mechanics, for top-down hyperelastic characterization of brain white matter tissue components, based on the directional homogenized responses of the tissue in the axial and transverse directions. The white matter is considered as a transversely isotropic neo-Hookean composite made of unidirectional distribution of axonal fibers within the extracellular matrix. First, two homogenization formulations are derived for the homogenized axial and transverse shear moduli of the tissue, based on definition of the strain energy density function. Next, the rule of mixtures and... 

    Can the body slope of interference screw affect initial stability of reconstructed anterior cruciate ligament?: An in-vitro investigation

    , Article BMC Musculoskeletal Disorders ; Volume 22, Issue 1 , 2021 ; 14712474 (ISSN) Daneshvarhashjin, N ; Chizari, M ; Mortazavi, J ; Rouhi, G ; Sharif University of Technology
    BioMed Central Ltd  2021
    Background: Superior biomechanical performance of tapered interference screws, compared with non-tapered screws, with reference to the anterior cruciate ligament (ACL) reconstruction process, has been reported in the literature. However, the effect of tapered interference screw’s body slope on the initial stability of ACL is poorly understood. Thus, the main goal of this study was to investigate the effect of the interference screw’s body slope on the initial stability of the reconstructed ACL. Methods: Based on the best screw-bone tunnel diameter ratios in non-tapered screws, two different tapered interference screws were designed and fabricated. The diameters of both screws were equal to... 

    Allergic rhinitis impairs working memory in association with drop of hippocampal – Prefrontal coupling

    , Article Brain Research ; Volume 1758 , 2021 ; 00068993 (ISSN) Salimi, M ; Ghazvineh, S ; Nazari, M ; Dehdar, K ; Garousi, M ; Zare, M ; Tabasi, F ; Jamaati, H ; Salimi, A ; Barkley, V ; Mirnajafi Zadeh, J ; Raoufy, M. R ; Sharif University of Technology
    Elsevier B.V  2021
    Allergic rhinitis (AR) is a chronic inflammatory disease frequently associated with a deficit in learning and memory. Working memory is an important system for decision making and guidance, which depends on interactions between the ventral hippocampus (vHipp) and the prelimbic prefrontal cortex (plPFC). It is still unclear whether AR influences the activity and coupling of these brain areas, which consequently may impair working memory. The current study aimed to examine alterations of the vHipp-plPFC circuit in a rat model of AR. Our results show decreased working memory performance in AR animals, accompanied by a reduction of theta and gamma oscillations in plPFC. Also, AR reduces... 

    Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study

    , Article International Journal of Pharmaceutics ; Volume 592 , 2021 ; 03785173 (ISSN) Shamloo, A ; Aghababaie, Z ; Afjoul, H ; Jami, M ; Bidgoli, M. R ; Vossoughi, M ; Ramazani, A ; Kamyabhesari, K ; Sharif University of Technology
    Elsevier B.V  2021
    In this study, physically cross-linked hydrogels were developed by freezing-thawing method while different concentrations of honey were included into the hydrogels for accelerated wound healing. The hydrogel was composed of chitosan, polyvinyl alcohol (PVA), and gelatin with the ratio of 2:1:1 (v/v), respectively. Further, the effect of honey concentrations on antibacterial properties, and cell behavior was investigated. In vivo studies, including wound healing mechanism using rat model and histological analysis of section tissue samples were performed. The results illustrated that the incorporation of honey in hydrogels increased the ultimate strain of hydrogels approximately two times,... 

    Three-dimensional simulation of urine concentrating mechanism in a functional unit of rat outer medulla. I. Model structure and base case results

    , Article Mathematical Biosciences ; Vol. 258 , 2014 , pp. 44-56 ; ISSN: 00255564 Sohrabi, S ; Saidi, M. S ; Saadatmand, M ; Banazadeh, M. H ; Firoozabadi, B ; Sharif University of Technology
    The urine formation and excretion system have long been of interest for mathematicians and physiologists to elucidate the obscurities within the process happens in renal tissue. In this study, a novel three-dimensional approach is utilized for modeling the urine concentrating mechanism in rat renal outer medulla which is essentially focused on demonstrating the significance of tubule's architecture revealed in anatomic studies and physiological literature. Since nephrons and vasculatures work interdependently through a highly structured arrangement in outer medulla which is dominated by vascular bundles, a detailed functional unit is proposed based on this specific configuration....