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    Type V collagen in scar tissue regulates the size of scar after heart injury

    , Article Cell ; Volume 182, Issue 3 , 2020 , Pages 545-562.e23 Yokota, T ; McCourt, J ; Ma, F ; Ren, S ; Li, S ; Kim, T. H ; Kurmangaliyev, Y. Z ; Nasiri, R ; Ahadian, S ; Nguyen, T ; Tan, X. H. M ; Zhou, Y ; Wu, R ; Rodriguez, A ; Cohn, W ; Wang, Y ; Whitelegge, J ; Ryazantsev, S ; Khademhosseini, A ; Teitell, M. A ; Chiou, P. Y ; Birk, D. E ; Rowat, A. C ; Crosbie, R. H ; Pellegrini, M ; Seldin, M ; Lusis, A. J ; Deb, A ; Sharif University of Technology
    Cell Press  2020
    Scar tissue size following myocardial infarction is an independent predictor of cardiovascular outcomes, yet little is known about factors regulating scar size. We demonstrate that collagen V, a minor constituent of heart scars, regulates the size of heart scars after ischemic injury. Depletion of collagen V led to a paradoxical increase in post-infarction scar size with worsening of heart function. A systems genetics approach across 100 in-bred strains of mice demonstrated that collagen V is a critical driver of postinjury heart function. We show that collagen V deficiency alters the mechanical properties of scar tissue, and altered reciprocal feedback between matrix and cells induces... 

    Three-dimensional simulation of urine concentrating mechanism in a functional unit of rat outer medulla. I. Model structure and base case results

    , Article Mathematical Biosciences ; Vol. 258 , 2014 , pp. 44-56 ; ISSN: 00255564 Sohrabi, S ; Saidi, M. S ; Saadatmand, M ; Banazadeh, M. H ; Firoozabadi, B ; Sharif University of Technology
    The urine formation and excretion system have long been of interest for mathematicians and physiologists to elucidate the obscurities within the process happens in renal tissue. In this study, a novel three-dimensional approach is utilized for modeling the urine concentrating mechanism in rat renal outer medulla which is essentially focused on demonstrating the significance of tubule's architecture revealed in anatomic studies and physiological literature. Since nephrons and vasculatures work interdependently through a highly structured arrangement in outer medulla which is dominated by vascular bundles, a detailed functional unit is proposed based on this specific configuration.... 

    The influence of temperature alterations on eccentric contraction-induced isometric force and desmin loss in ratmedial gastrocnemius muscle

    , Article Journal of Medical Sciences ; Volume 8, Issue 2 , 2008 , Pages 162-169 ; 16824474 (ISSN) Vasaghi Gharamaleki, B ; Keshavarz, M ; Gharibzadeh, S ; Sotodeh, M ; Marvi, H ; Mosayebnejad, J ; Ebrahimi Takamjani, I ; Sharif University of Technology
    In this study isolated perfused rat muscle was used to examine the direct effect of temperature changes on the eccentric contraction-induced force and desmin loss. The left medial gastrocnemius muscle was separated and the entire lower limb was transferred into a prewarmed (35°C) organ bath. Temperature was adjusted to 31 or 39°C before and during eccentric contractions. Maximal isometric force and desmin loss were measured after 15 isometric or eccentric contractions. According to our data, organ bath temperature changes before or during eccentric contractions had no significant effect on force loss. However, a strong correlation between desmin loss and temperature changes before (r = 0.93,... 

    Temporal activation of LRH-1 and RAR-γ in human pluripotent stem cells induces a functional naïve-like state

    , Article EMBO Reports ; Volume 21, Issue 10 , 2020 Taei, A ; Kiani, T ; Taghizadeh, Z ; Moradi, S ; Samadian, A ; Mollamohammadi, S ; Sharifi Zarchi, A ; Guenther, S ; Akhlaghpour, A ; Asgari Abibeiglou, B ; Najar Asl, M ; Karamzadeh, R ; Khalooghi, K ; Braun, T ; Hassani, S. N ; Baharvand, H ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Naïve pluripotency can be established in human pluripotent stem cells (hPSCs) by manipulation of transcription factors, signaling pathways, or a combination thereof. However, differences exist in the molecular and functional properties of naïve hPSCs generated by different protocols, which include varying similarities with pre-implantation human embryos, differentiation potential, and maintenance of genomic integrity. We show here that short treatment with two chemical agonists (2a) of nuclear receptors, liver receptor homologue-1 (LRH-1) and retinoic acid receptor gamma (RAR-γ), along with 2i/LIF (2a2iL) induces naïve-like pluripotency in human cells during reprogramming of fibroblasts,... 

    Synergy between hemagglutinin 2 (HA2) subunit of influenza fusogenic membrane glycoprotein and oncolytic Newcastle disease virus suppressed tumor growth and further enhanced by Immune checkpoint PD-1 blockade

    , Article Cancer Cell International ; Volume 20, Issue 1 , August , 2020 Miri, S. M ; Ebrahimzadeh, M. S ; Abdolalipour, E ; Yazdi, M ; Hosseini Ravandi, H ; Ghaemi, A ; Sharif University of Technology
    BioMed Central Ltd  2020
    Background: Newcastle disease virus (NDV) has shown noticeable oncolytic properties, especially against cervical cancer. However, in order to improve the spread rate and oncotoxicity of the virus, employment of other therapeutic reagents would be helpful. It has been shown that some viral fusogenic membrane glycoproteins (FMGs) could facilitate viral propagation and increase the infection rate of tumor cells by oncolytic viruses. Additionally, immune checkpoint blockade has widely been investigated for its anti-tumor effects against several types of cancers. Here, we investigated for the first time whether the incorporation of influenza hemagglutinin-2 (HA2) FMG could improve the oncolytic... 

    Simulation of blood oxygenation in capillary membrane oxygenators using modified sulfite solution

    , Article Biophysical Chemistry ; Vol. 195, issue , Dec , 2014 , p. 8-15 Tabesh, H ; Amoabediny, G ; Rasouli, A ; Ramedani, A ; Poorkhalil, A ; Kashefi, A ; Mottaghy, K ; Sharif University of Technology
    Blood oxygenation is the main performance characteristic of capillary membrane oxygenators (CMOs). Handling of natural blood in in vitro investigations of CMOs is quite complex and time-consuming. Since the conventional blood analog fluids (e.g. water/glycerol) lack a substance with an affinity to capture oxygen comparable to hemoglobin's affinity, in this study a novel approach using modified sulfite solution is proposed to address this challenge. The solution comprises sodium sulfite as a component, simulating the role of hemoglobin in blood oxygenation. This approach is validated by OTR (oxygen transfer rate) measured using native porcine blood, in two types of commercially available... 

    SAR thresholds for electromagnetic exposure using functional thermal dose limits

    , Article International Journal of Hyperthermia ; Volume 34, Issue 8 , 2018 , Pages 1248-1254 ; 02656736 (ISSN) Adibzadeh, F ; Paulides, M. M ; Van Rhoon, G. C ; Sharif University of Technology
    Taylor and Francis Ltd  2018
    Background and purpose: To protect against any potential adverse effects to human health from localised exposure to radio frequency (100 kHz–3 GHz) electromagnetic fields (RF EMF), international health organisations have defined basic restrictions on specific absorption rate (SAR) in tissues. These exposure restrictions incorporate safety factors which are generally conservative so that exposures that exceed the basic restrictions are not necessarily harmful. The magnitude of safety margin for various exposure scenarios is unknown. This shortcoming becomes more critical for medical applications where the safety guidelines are required to be relaxed. The purpose of this study was to quantify... 

    Role of endurance training in preventing pathological hypertrophy via large tumor suppressor (LATS) changes

    , Article Iranian Heart Journal ; Volume 20, Issue 3 , 2019 , Pages 52-59 ; 17357306 (ISSN) Tabrizi, A ; Soori, R ; Choobineh, S ; Gholipour, M ; Sharif University of Technology
    Iranian Heart Association  2019
    Background: One of the negative effects of cardiac sympathetic hyperactivity is pathologic hypertrophy. Recent studies have indicated that large tumor suppressor (LATS) is one of the molecules which play a critical role in cardiomyocyte apoptosis. Considering the preventive role of exercise training, we evaluated the effects of endurance training on LATS gene expression and its upstream pathway in the present study. Methods: Eighteen male Wistar rats were randomly divided into 2 groups: Endurance and control. Endurance training was performed for 8 weeks, 1 hour per day, and 6 days per week on the treadmill at a 15° inclination. Pathologic hypertrophy was induced with the injection of 3... 

    Real-time simulation of the nonlinear visco-elastic deformations of soft tissues

    , Article International Journal of Computer Assisted Radiology and Surgery ; Volume 6, Issue 3 , 2011 , Pages 297-307 ; 18616410 (ISSN) Basafa, E ; Farahmand, F ; Sharif University of Technology
    Purpose: Mass-spring-damper (MSD) models are often used for real-time surgery simulation due to their fast response and fairly realistic deformation replication. An improved real time simulation model of soft tissue deformation due to a laparoscopic surgical indenter was developed and tested. Method: The mechanical realization of conventional MSD models was improved using nonlinear springs and nodal dampers, while their high computational efficiency was maintained using an adapted implicit integration algorithm. New practical algorithms for model parameter tuning, collision detection, and simulation were incorporated. Results: The model was able to replicate complex biological soft tissue... 

    Quantitative in vivo microsampling for pharmacokinetic studies based on an integrated solid-phase microextraction system

    , Article Analytical Chemistry ; Volume 79, Issue 12 , 2007 , Pages 4507-4513 ; 00032700 (ISSN) Zhang, X ; Eshaghi, A ; Musteata, F. M ; Ouyang, G ; Pawliszyn, J ; Sharif University of Technology
    An integrated microsampling approach based on solid-phase microextraction (SPME) was developed to provide a complete solution to highly efficient and accurate pharmacokinetic studies. The microsampling system included SPME probes that are made of poly(ethylene glycol) (PEG) and C18-bonded silica, a fast and efficient sampling strategy with accurate kinetic calibration, and a high-throughput desorption device based on a modified 96-well plate. The sampling system greatly improved the quantitative capability of SPME in two ways. First, the use of the C18-bonded silica/PEG fibers minimized the competition effect from analogues of the target analytes in a complicated sample matrix such as blood... 

    Preparation and biological evaluation of radiolabeled-folate embedded superparamagnetic nanoparticles in wild-type rats

    , Article Journal of Radioanalytical and Nuclear Chemistry ; Volume 287, Issue 1 , January , 2011 , Pages 119-127 ; 02365731 (ISSN) Jalilian, A. R ; Hosseini Salekdeh, S. L ; Mahmoudi, M ; Yousefnia, H ; Majdabadi, A ; Pouladian, M ; Sharif University of Technology
    In this study, superparamagnetic iron oxide nanoparticles (SPION) embedded by folic acid (SPION-folate) were prepared by a modified co-precipitation method. The structure, size, morphology, magnetic property and relaxivity of the SPION-folate were characterized systematically by means of XRD, VSM, HRSEM and TEM and the interaction between folate and iron oxide (Fe3O 4) was characterized by FT-IR. The particle size was shown to be ≈5-10 nm. To ensure biocompatibility, the interaction of these SPION with mouse connective tissue cells (adhesive) was investigated using an MTT assay. Consequently, gallium-67 labeled nanoparticles ([67Ga]-SPION-folate) were prepared using 67Ga with a high labeling... 

    Phenomenological tissue fracture modeling for an Endoscopic Sinus and Skull Base Surgery training system based on experimental data

    , Article Medical Engineering and Physics ; Volume 68 , 2019 , Pages 85-93 ; 13504533 (ISSN) Sadeghnejad, S ; Farahmand, F ; Vossoughi, G ; Moradi, H ; Mousa Sadr Hosseini, S ; Sharif University of Technology
    Elsevier Ltd  2019
    The ideal simulator for Endoscopic Sinus and Skull Base Surgery (ESSS)training must be supported by a physical model and provide repetitive behavior in a controlled environment. Development of realistic tissue models is a key part of ESSS virtual reality (VR)-based surgical simulation. Considerable research has been conducted to address haptic or force feedback and propose a phenomenological tissue fracture model for sino-nasal tissue during surgical tool indentation. Mechanical properties of specific sino-nasal regions of the sheep head have been studied in various indentation and relaxation experiments. Tool insertion at different indentation rates into coronal orbital floor (COF)tissue is... 

    Oncolytic newcastle disease virus delivered by mesenchymal stem cells-engineered system enhances the therapeutic effects altering tumor microenvironment

    , Article Virology Journal ; Volume 17, Issue 1 , 2020 Keshavarz, M ; Ebrahimzadeh, M. S ; Miri, S. M ; Dianat Moghadam, H ; Ghorbanhosseini, S. S ; Mohebbi, S. R ; Keyvani, H ; Ghaemi, A ; Sharif University of Technology
    BioMed Central Ltd  2020
    Background: Human papillomavirus (HPV)-associated malignancy remain a main cause of cancer in men and women. Cancer immunotherapy has represented great potential as a new promising cancer therapeutic approach. Here, we report Mesenchymal stem cells (MSCs) as a carrier for the delivery of oncolytic Newcastle disease virus (NDV) for the treatment of HPV-associated tumor. Methods: For this purpose, MSCs obtained from the bone marrow of C57BL mice, then cultured and characterized subsequently by the flow cytometry analysis for the presence of cell surface markers. In this study, we sought out to determine the impacts of MSCs loaded with oncolytic NDV on splenic T cell and cytokine immune... 

    Nonlinear mechanics of soft composites: hyperelastic characterization of white matter tissue components

    , Article Biomechanics and Modeling in Mechanobiology ; Volume 19, Issue 3 , 2020 , Pages 1143-1153 Yousefsani, S. A ; Shamloo, A ; Farahmand, F ; Sharif University of Technology
    Springer  2020
    This paper presents a bi-directional closed-form analytical solution, in the framework of nonlinear soft composites mechanics, for top-down hyperelastic characterization of brain white matter tissue components, based on the directional homogenized responses of the tissue in the axial and transverse directions. The white matter is considered as a transversely isotropic neo-Hookean composite made of unidirectional distribution of axonal fibers within the extracellular matrix. First, two homogenization formulations are derived for the homogenized axial and transverse shear moduli of the tissue, based on definition of the strain energy density function. Next, the rule of mixtures and... 

    Modeling, simulation, and optimal initiation planning for needle insertion into the liver

    , Article Journal of Biomechanical Engineering ; Volume 132, Issue 4 , 2010 ; 01480731 (ISSN) Sharifi Sedeh, R ; Ahmadian, M. T ; Janabi Sharifi, F ; Sharif University of Technology
    Needle insertion simulation and planning systems (SPSs) will play an important role in diminishing inappropriate insertions into soft tissues and resultant complications. Difficulties in SPS development are due in large part to the computational requirements of the extensive calculations in finite element (FE) models of tissue. For clinical feasibility, the computational speed of SPSs must be improved. At the same time, a realistic model of tissue properties that reflects large and velocity-dependent deformations must be employed. The purpose of this study is to address the aforementioned difficulties by presenting a cost-effective SPS platform for needle insertions into the liver. The study... 

    Investigation of the effect of high +Gz accelerations on human cardiac function

    , Article Journal of the Mechanical Behavior of Biomedical Materials ; Volume 27 , 2013 , Pages 54-63 ; 17516161 (ISSN) Jamshidi, M ; Ahmadian, M. T ; Sharif University of Technology
    This study investigates the effect of body acceleration on human cardiac function. Finite element analysis is conducted to simulate geometrical and mechanical properties of human heart. Heart geometrical modeling in three-dimension is performed by segmentation of cardiac MRI images. The nonlinear mechanical behavior of myocardium is modeled by Mooney-Rivlin, Polynomial, Ogden and Yeoh hyperelastic material models. Stress-strain curves of myocardial tissue are obtained from experimental compression tests on bovine heart samples. The experimental results are employed for the evaluation of material coefficients by the nonlinear least squares method. Among hyperelastic models, the Yeoh model... 

    Hyperthermia of breast cancer tumor using graphene oxide-cobalt ferrite magnetic nanoparticles in mice

    , Article Journal of Drug Delivery Science and Technology ; Volume 65 , 2021 ; 17732247 (ISSN) Hatamie, S ; Balasi, Z. M ; Ahadian, M. M ; Mortezazadeh, T ; Shams, F ; Hosseinzadeh, S ; Sharif University of Technology
    Editions de Sante  2021
    Herein, the graphene oxide (GO)/cobalt ferrite nanoparticles were used to apply the heat treatment on the breast cancer cell line of MCF7. The synthesized nanoparticles were evaluated before in vitro and in vivo studies, using transmission electron microscopy (TEM), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), X-ray photoelectron spectroscopy (XPS), thermal property and relaxivity measurement. The nanoparticles showed a diameter of 5 nm with the ferrimagnetic property. Also, the nanoparticles were well distributed on the GO nanosheets. The related peaks of cobalt ferrite nanoparticles were approved by using XRD and XPS assays. During the in vitro investigations, IC50 with... 

    Homozygous mutations in C14orf39/SIX6OS1 cause non-obstructive azoospermia and premature ovarian insufficiency in humans

    , Article American Journal of Human Genetics ; Volume 108, Issue 2 , 2021 , Pages 324-336 ; 00029297 (ISSN) Fan, S ; Jiao, Y ; Khan, R ; Jiang, X ; Javed, A. R ; Ali, A ; Zhang, H ; Zhou, J ; Naeem, M ; Murtaza, G ; Li, Y ; Yang, G ; Zaman, Q ; Zubair, M ; Guan, H ; Zhang, X ; Ma, H ; Jiang, H ; Ali, H ; Dil, S ; Shah, W ; Ahmad, N ; Zhang, Y ; Shi, Q ; Sharif University of Technology
    Cell Press  2021
    Human infertility is a multifactorial disease that affects 8%–12% of reproductive-aged couples worldwide. However, the genetic causes of human infertility are still poorly understood. Synaptonemal complex (SC) is a conserved tripartite structure that holds homologous chromosomes together and plays an indispensable role in the meiotic progression. Here, we identified three homozygous mutations in the SC coding gene C14orf39/SIX6OS1 in infertile individuals from different ethnic populations by whole-exome sequencing (WES). These mutations include a frameshift mutation (c.204_205del [p.His68Glnfs∗2]) from a consanguineous Pakistani family with two males suffering from non-obstructive... 

    Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study

    , Article International Journal of Pharmaceutics ; Volume 592 , 2021 ; 03785173 (ISSN) Shamloo, A ; Aghababaie, Z ; Afjoul, H ; Jami, M ; Bidgoli, M. R ; Vossoughi, M ; Ramazani, A ; Kamyabhesari, K ; Sharif University of Technology
    Elsevier B.V  2021
    In this study, physically cross-linked hydrogels were developed by freezing-thawing method while different concentrations of honey were included into the hydrogels for accelerated wound healing. The hydrogel was composed of chitosan, polyvinyl alcohol (PVA), and gelatin with the ratio of 2:1:1 (v/v), respectively. Further, the effect of honey concentrations on antibacterial properties, and cell behavior was investigated. In vivo studies, including wound healing mechanism using rat model and histological analysis of section tissue samples were performed. The results illustrated that the incorporation of honey in hydrogels increased the ultimate strain of hydrogels approximately two times,... 

    Fabrication and evaluation of a bilayer hydrogel-electrospinning scaffold prepared by the freeze-gelation method

    , Article Journal of Biomechanics ; Volume 98 , 2020 Kamali, A ; Shamloo, A ; Sharif University of Technology
    Elsevier Ltd  2020
    This study presents a bilayer structure as a skin scaffold comprised of an electrospun sheet layer made of polycaprolactone and polyvinil alcohol and a porous hydrogel layer made of chitosan and gelatin. The hydrogel layer was fabricated by employing the freeze-gelation technique. The bilayer structure was achieved by pouring the hydrogel solution on the electrospun sheet at the bottom of a mold followed by the freeze-gelation technique to obtain a porous structure in the hydrogel. The hydrogel and hydrogel-electrospun samples were characterized by scanning electron microscopy, swelling, tensile strength, in vitro and in vivo analyses. From a mechanical strength standpoint, the combination...