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Microcarriers Development for Stem Cells Cultivation

Karimian Shamsabadi, Mohammad | 2014

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 46489 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohre; Yaghmaei, Soheyla
  7. Abstract:
  8. In general, repair and regeneration of damaged tissues or lost organ is base definition of a technology named tissue engineering. Tissue engineering technology provides innovative path to achieve better medical treatment, including different methods which manipulate natural or synthetic biomaterials mimicking stem cells environmental condition inside the body, in order to facilitate desired cell expansion and differentiation. Recently, application of cell carriers in form of microcarriers which are injectable, biodegradable and biocompatible have attracted significant attention. Due to increasing effectiveness in animal cell culture.
    The aim of this work was fabrication of chitosan-gelatin porous microcarriers as well as expansion of mesenchymal stem cell on these microcarriers and finally optimization of chitosan-gelatin composition and culture condition, in order to provide proper culture coditions for cells to retain the pluripotency and desired growth. After microcarriers fabrication, stem cells were seeded onto them and expanded under dynamic culture system. Proper composition of chitosan and gelatin, stem cells adherent and expansion were investigated.
    Results showed that microcarriers which contained 2 % chitosan(w/w) and 24 gelatin (w/w) had the best performance for adherence and expansion of stem cells. The microcarriers had proper morphology and physical stiffness. In comparison with the commercial microcarriers, chitosan-gelatin microcarriers showed suitable efficiency
  9. Keywords:
  10. Cell Culture ; Stem Cell ; Microcarrier ; Tissue Engineering

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