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Embryonic stem cells maintain an undifferentiated state on dendrimer-immobilized surface with D-glucose display

Mashayekhan, S ; Sharif University of Technology | 2011

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  1. Type of Document: Article
  2. DOI: 10.3390/polym3042078
  3. Publisher: 2011
  4. Abstract:
  5. In serial passaging cultures of mouse embryonic stem (ES) cells, we employed a dendrimer-immobilized substrate that displayed D-glucose as a terminal ligand. The D-glucose-displaying dendrimer (GLU/D) surface caused the ES cells to form loosely attached spherical colonies, while those on a gelatin-coated surface formed flatter colonies that were firmly attached to the surface. Despite the morphological similarities between the colonies on the GLU/D surface and aggregates on a conventional bacteriological dish, immunostaining and RT-PCR analyses revealed the maintenance of cells within the spherical colonies on the GLU/D surface in an undifferentiated state with very low expressions of primitive endoderm markers. On the bacteriological dish, however, the cells within the aggregates showed a different cellular state with partial differentiation into the primitive endoderm lineage, and the expression level increased gradually along with the number of passages. These results indicate that the GLU/D surface can be a potential tool for controlling the ES cell morphology and then govern their self-renewal and fate
  6. Keywords:
  7. Cellular state ; D-glucose ; Embryonic stem cells ; ES cells ; Expression levels ; Glucose-displaying dendrimer surface ; Immunostaining ; Potential tool ; Primitive endoderm ; RT-PCR analysis ; Spherical colony ; Terminal ligands ; Aggregates ; Cell growth ; Dendrimers ; Glucose ; Spheres ; Stem cells ; Cell culture
  8. Source: Polymers ; Volume 3, Issue 4 , 2011 , Pages 2078-2087 ; 20734360 (ISSN)
  9. URL: http://www.mdpi.com/2073-4360/3/4/2078