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The characterization of proteins involved in toxic metal resistance of a bacterial strain isolated from mine site using a proteomic approach

Nemati, F ; Sharif University of Technology

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  1. Type of Document: Article
  2. Abstract:
  3. We investigated the response of a bacterial strain isolated from low grade complex zinc and lead sulfide mines to metals toxicity (i.e., zinc, manganese, nickel, cobalt, copper, arsenate, chromium, lead, and mercury). The bacterium was identified as a strain of Aciditiobacillus ferrooxidans. The isolate showed good resistance to most of the toxic metals. The proteomics approach was used to identify the differentially expressed proteins under heavy metal stress in this strain. Four of the differentially expressed proteins were identified as major outer membrane protein of A. ferrooxidans, ribulose bisphosphate carboxylase large subunit of A. ferrooxidans, putative DNA restriction methylase, and holo synthase (Syntrophus aciditrophicus). Their role in metal resistance is discussed. Copyright © Enviromedia
  4. Keywords:
  5. 2D PAGE ; Bioleaching ; Metal toxicity ; Bacterium ; DNA ; Enzyme activity ; Heavy metal ; Isolated population ; Leaching ; Mass spectrometry ; Mine ; Protein ; Proteomics ; Toxicity ; Acidithiobacillus ferrooxidans ; Bacteria (microorganisms) ; Syntrophus aciditrophicus
  6. Source: Pollution Research ; Volume 28, Issue 3 , 2009 , Pages 337-343 ; 02578050 (ISSN)