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Design and Fabrication of Biodegradable Polymeric Scaffold with nano-Bioglass for Osteoblast cell Growth

Razaghzadeh Bidgoli, Mina | 2017

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50735 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vossoughi, Manouchehr; Alemzadeh, Iran; Tamjid Shabesteri, Elnaz
  7. Abstract:
  8. Treatment of critical-size bone defects caused by sport injuries, accidents, trauma, infection, and osteoporosis remains a major clinical challenge. In order to repair or regenerate large bone defects, bioactive three-dimensional scaffolds play a key role due to their multilevel porous structure, high surface area, enhanced mass transport and diffusion. Many studies reported that macropore diameters greater than 500 µm can lead to vascularized bone tissue. In this study, a hierarchically porous composite scaffold was prepared. Hierarchically porous silk fibroin- bioactive glass composite and fibroin scaffold were fabricated with controlled architecture and interconnected structure with macro and micro pores by combining an indirect three-dimensional printing and freeze-drying method. Furthermore, the antibacterial activities of scaffolds were investigated using E. coli and S. aureus microorganisms. The results demonstrated that the hierarchical structure in this scaffolds were composed of two levels of pores on the order of 500-600 μm and 10-50 μm with a porosity of 90.5%. Scaffolds have been flexible mechanical properties in biological conditions of the body in such a way that no cracks in the walls of the scaffold were observed by loading force. The prepared silk fibroin-bioactive glass scaffold has antibacterial activities and zones of clearance was obvious around the scaffolds in the nutrient agar plate. Also, the scaffold’s biocampatibility properties, was investigated. It was found that the presence of nano-bioglass in the scaffold increased the compressive biocampatibility of silk fibroin scaffold up to 60%
  9. Keywords:
  10. Tissue Engineering ; Bone Scaffold ; Bioglass ; Fibroin ; Silk Fibroin ; Biodegradable Scaffold

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