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Study and Fabrication of a Multilayer Scaffold Containing Biological Agents for Skin Wounds Regeneration

Hajiabbas, Maryam | 2020

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  1. Type of Document: Ph.D. Dissertation
  2. Language: Farsi
  3. Document No: 53418 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Alemzadeh, Iran; Vossoughi, Manouchehr; Shamloo, Amir
  7. Abstract:
  8. In recent years, it is expected that the fabrication of multilayer scaffolds and the use of different methodologies in one product can be a new progressing method in skin substitute production. Accordingly, this project aims to fabricate a bilayered composite scaffold with a combination of hydrogel and electrospinning method. We have tried to prepare a scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) without using corrosive solvents and toxic crosslinking agents as a scaffold and drug delivery system. As different biological, chemical, physical, and mechanical factors play a vital role in the healing process, we have characterized the proposed scaffold via DSC, TGA, XRD, FTIR, SEM, and tensile properties. Moreover, release profile and release kinetic models for simvastatin (SIM) and bovine serum albumin (BSA) from different parts of the composite scaffold have been investigated. The biocompatibility and bioactivity of the drug and protein-loaded scaffolds have also been studied by cellular and antibacterial analysis. The SEM images demonstrated that the fabrication method of the proposed scaffolds is suitable to mimic the nano and microstructure of the skin tissue. The mechanical analysis showed that the composite scaffold with 15-98% elongation and 0/5-13 Mpa elastic modulus is a suitable candidate for skin tissue engineering applications. SIM and BSA's release profile demonstrated that the fabrication method, the type of fiber, and the hybrid structure are essential in their release behavior. The data indicated that the first-order model had a suitable R2 (0/9687) value to fit with BSA's release data from SF fiber. In contrast, the Korsmeyer-Peppas (Power-law) model is more fit for the release data of loaded BSA in G fibers (R2= 0/8743). The results also showed a sustainable and controlled release of these active agents from nanofibrous hydrogel scaffold by selecting an appropriate place to encapsulate them (for instance, zero-order kinetic for encapsulated protein in SF fibers). Moreover, the cellular analysis indicated suitable biocompatibility and bioactivity of the prepared scaffolds for biomedical engineering applications. Further, the results showed a favorable adipose-derived mesenchymal stem cell’s (AMSC) attachment, proliferation, distribution, and infiltration within this novel hydrogel-electrospun composite. The results showed that viability of the seeded cells on the proposed scaffolds was 200% higher than higher than the tissue culture plate. The antibacterial analysis also indicated suitable bioactivity of the drug-loaded scaffolds. In conclusion, such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new candidate for skin and different tissue engineering applications as well as a controlled release of more than one active agent in one drug delivery system
  9. Keywords:
  10. Oxidized Alginate ; Gelatin ; Silk Fibroin ; Skin Tissue Engineering ; Hydrogel ; Drug Delivery ; Three Dimentional Electrospun ; Porous Hydrogel-Electrospun Scaffold ; Skin Regeneration ; Hydrogel Scaffold

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