Loading...

Mechanical characteristics of SPG-178 hydrogels: Optimizing viscoelastic properties through microrheology and response surface methodology

Seyedkarimi, M. S ; Sharif University of Technology | 2020

453 Viewed
  1. Type of Document: Article
  2. DOI: 10.29252/ibj.24.2.110
  3. Publisher: Pasteur Institute of Iran , 2020
  4. Abstract:
  5. Background: SApeptides have growing applications in tissue engineering and regenerative medicine. The application of SApeptide-based hydrogels depends strongly on their viscoelastic properties. Optimizing the properties is of importance in tuning the characteristics of the hydrogels for a variety of applications. Methods: In this study, we employed statistical modeling, conducted with the RSM and particle tracking microrheology, to investigate the effects of self-assembling SPG-178 peptide and added NaCl salt concentrations and milieu type (DI water or blood serum) on the viscoelastic properties of SPG-178 hydrogels. A central composite RSM model was employed for finding the optimum value of the parameters to achieve the highest storage modulus and the lowest tan δ. Results: Viscoelastic properties of each sample, including storage modulus, loss modulus, and tan δ, were determined. Storage modulus and tan δ were modeled, accounting for the impact of the SPG-178 peptide and NaCl concentrations and milieu type on the viscoelastic properties. It was found that the SPG-178 hydrogel storage modulus was positively influenced by the SPG-178 peptide concentration and the serum. Conclusion: A combination of microrheology and RSM is a useful test method for statistical modeling and analysis of rheological behavior of solid-like gels, which could be applied in various biomedical applications such as hemostasis. © 2020, Pasteur Institute of Iran. All rights reserved
  6. Keywords:
  7. Regenerative medicine ; Rheology ; SPG-178 ; Tissue engineering ; Peptide ; Viscoelastic substance ; Chemistry ; Hydrogel ; physiological stress ; physiology ; procedures ; statistical model ; Hydrogels ; Models, Statistical ; Peptides ; Regenerative medicine ; Sodium chloride ; Stress, physiological ; Tissue engineering ; Viscoelastic substances
  8. Source: Iranian Biomedical Journal ; Volume 24, Issue 2 , 2020 , Pages 110-118
  9. URL: http://ibj.pasteur.ac.ir/browse.php?a_id=2953&slc_lang=en&sid=1&printcase=1&hbnr=1&hmb=1