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Sol-gel-based solid-phase microextraction and gas chromatography-mass spectrometry determination of dextromethorphan and dextrorphan in human plasma

Bagheri, H ; Sharif University of Technology | 2005

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  1. Type of Document: Article
  2. DOI: 10.1016/j.jchromb.2004.12.031
  3. Publisher: 2005
  4. Abstract:
  5. A novel solid-phase microextraction (SPME) method was developed for isolation of dextromethorphan (DM) and its main metabolite dextrorphan (DP) from human plasma followed by GC-MS determination. Three different polymers, poly(dimethylsiloxane) (PDMS), poly(ethylenepropyleneglycol) monobutyl ether (Ucon) and polyethylene glycol (PEG) were synthesized as coated fibers using sol-gel methodologies. DP was converted to its acetyl-derivative prior to extraction and subsequent determination. The porosity of coated fibers was examined by SEM technique. Effects of different parameters such as fiber coating type, extraction mode, agitation method, sample volume, extraction time, and desorption condition, were investigated and optimized. The method is rapid, simple, easy and inexpensive and offers high sensitivity and reproducibility. The limits of detection are 0.010 and 0.015 ng/ml for DM and DP, respectively. The precisions for both analytes are below 5% (n = 5). The correlation coefficient was satisfactory (r2 > 0.99) for both DM and DP. Linear ranges were obtained from 0.03 ng/ml to 2 μg/ml for DM and from 0.05 ng/ml to 2 μg/ml for DP. © 2004 Elsevier B.V. All rights reserved
  6. Keywords:
  7. Gas chromatography ; Porosity ; Scanning electron microscopy ; Sol-gels ; Dextromethorphan (DM) ; Dextrorphan (DP) ; Poly(dimethylsiloxane) (PDMS) ; Solid-phase microextraction (SPME) ; Plasmas ; Dimeticone ; Macrogol ; Aqueous solution ; Correlation analysis ; Desorption ; Drug coating ; Extraction ; Mass spectrometry ; Methodology ; Priority journal ; Solid phase microextraction ; Dextromethorphan ; Dextrorphan ; Dimethylpolysiloxanes ; Ethers ; Gas Chromatography-Mass Spectrometry ; Humans ; Hydrogen-Ion Concentration ; Microscopy, Electron, Scanning ; Phase Transition ; Polyethylene Glycols ; Reproducibility of Results ; Sensitivity and Specificity ; Silicones ; Solutions ; Temperature
  8. Source: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences ; Volume 818, Issue 2 , 2005 , Pages 147-157 ; 15700232 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S157002320401058X