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alamzadeh--eiran
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Measurement of Partition Coefficient of L-Lysine HCl Amino Acid in Polymer-Salt Aqueous Two-Phase Systems
, M.Sc. Thesis Sharif University of Technology ; Vossoughi, Manouchehr (Supervisor) ; Alamzadeh, Eiran (Supervisor)
Abstract
The partition coefficients of L-Lysine HCL were measured in polymer-salt aqueous two-phase systems. These systems contain polyethylene glycol with nominal molecular weight of 4000 or 10000 and two different salts (di-potassium hydrogen phosphate or tri-sodium citrate 5,5-hydrate). The experimental data were obtained at temperatures 293.15, 298.15 and 303.15 K. The effects of temperature, pH, polymer and salt concentrations, polymer molecular weight and salt type on the partitioning of L-Lysine HCL were also studied. The results showed that salt concentration has a significant effect on the partition coefficient while temperature has a low effect. The experimental partition coefficients of...
In vitro release behavior and cytotoxicity of doxorubicin-loaded gold nanoparticles in cancerous cells
, Article Biotechnology Letters ; Volume 32, Issue 5 , May , 2010 , Pages 649-654 ; 01415492 (ISSN) ; Vossoughi, M ; Alamzadeh, I ; Sharif University of Technology
2010
Abstract
Doxorubicin (DOX), a common cancer chemotherapeutics, was conjugated to folate-modified thiolated-polyethylene glycol-functionalized gold nanoparticles. The in vitro, controlled release behavior of DOX-loaded gold nanoparticles was observed using porous dialysis membranes (cut-off = 2 kDa). DOX-loaded gold nanoparticles had higher cytotoxicity for folate-receptor-positive cells (KB cells) compared to folate-receptor-negative cells (A549 cells) which were 48 and 62% viable for 10 μM doxorubicin, respectively. This indicates the potential of these nano-carriers for targeted-delivery. In addition, healthy cell viability was 69% for 10 μM free doxorubicin whereas for the same content of drug in...
Synthesis of folate-modified, polyethylene glycol-functionalized gold nanoparticles for targeted drug delivery
, Article Journal of Dispersion Science and Technology ; Volume 31, Issue 4 , 2010 , Pages 492-500 ; 01932691 (ISSN) ; Vosoughi, M ; Alamzadeh, I ; Tavakoli, A ; Sharif University of Technology
2010
Abstract
The chemical synthesis and in vitro drug delivery response of folate-modified, polyethylene glycol-functionalized gold nanoparticles were studied. Lysine-capped gold nanoparticles were first prepared. Subsequently, the widely used anticancer agent doxorubicin (DOX) was successfully attached to the surface of folate-modified, polyethylene glycol-functionalized gold nanoparticles. Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) were used to confirm the functionalization and conjugation steps in the chemical synthesis. The DOX-loading efficiency determined by UV-vis spectrometer was 80%. Drug release experiments displayed a controlled-release behavior...
Experimental Investigation on Growth and Control of Animal Cells Attached on Scaffold
, M.Sc. Thesis Sharif University of Technology ; Vossoughi, Manoochehr (Supervisor) ; Alamzadeh, Iran (Co-Advisor)
Abstract
Major bone defects resulted from diseases or hurts that can not heal naturally, requaire bone transplantation. golden standard for transplantation is autologus one but it has some disad-vantages such as economic burden, the lack of graft source, surgery on patient, and so on. so exploring altrnative ways led to exploit stem cells.traditionally bone marrow mesenchymal stem cells (MSCs) used for stem cell-bsaed bone regeneration. bone marrow aspiration to aquaire stem cells is painfull and give a population of cells such as hematopoetic cells that requaire to seperate and can not be handled in first passages. MSCs can be substituted by multipotent adipose tissue-derived stem cells (ADSCs) in...
Evaluation of Process Partitioning of Enzyme in Polymer-Salt Aqueous Two-PHase Systems
, M.Sc. Thesis Sharif University of Technology ; Alamzadeh, Iran (Supervisor) ; Vossoughi, Manouchehr (Supervisor) ; Pazuki, Gholamreza (Supervisor)
Abstract
In this research, the amount of partitioning Invertase to be evaluated in nine systems, these systems contain Peg 2000,Peg 4000 & Peg 6000 with three different salt.In first, Binodal curve for nine systems to be drown by cloudy point method, then five point be selected in each system such as sample feed. Invertase in every systems to be solved in constant weight fraction. The analysis of every phasesto be achieved by Merchuk method. For correlation data of this method to be used by Othmer-Tobias method and then using equation Mrchvk ensure the accuracy of phase analysis , analysis of the results obtained with the UV method was studied.Accuracy of UV analysis was studied by using lattice...
Simulation, Evaluation and Fabrication of a Two-step Microfluidic System to Separate Circulating Tumor Cells
, M.Sc. Thesis Sharif University of Technology ; Vosoughi, Manouchehr (Supervisor) ; Alamzadeh, Iran (Supervisor)
Abstract
Cancer is an abnormal growth of cells which is one of the major cause of worldwide’s mortality. Circulating Tumor Cells (CTCs) are rare cancer cells released from the primary or metastatic tumors and transported though the peripheral circulatory system and spreads in body and seize healthy organs. Early detection of CTCs can help in overtaking the cancer, hence isolation of CTCs is an essential step for many therapeutics. In spite of its clinical potential, the isolation and detection of CTCs has been a challenging task due to its rare presence amongst other blood cells (as low as 1–10 CTCs per billions of blood cells), similarity to white blood cell in size and also variability in terms of...