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In vitro release behavior and cytotoxicity of doxorubicin-loaded gold nanoparticles in cancerous cells

Asadishad, B ; Sharif University of Technology | 2010

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  1. Type of Document: Article
  2. DOI: 10.1007/s10529-010-0208-x
  3. Publisher: 2010
  4. Abstract:
  5. Doxorubicin (DOX), a common cancer chemotherapeutics, was conjugated to folate-modified thiolated-polyethylene glycol-functionalized gold nanoparticles. The in vitro, controlled release behavior of DOX-loaded gold nanoparticles was observed using porous dialysis membranes (cut-off = 2 kDa). DOX-loaded gold nanoparticles had higher cytotoxicity for folate-receptor-positive cells (KB cells) compared to folate-receptor-negative cells (A549 cells) which were 48 and 62% viable for 10 μM doxorubicin, respectively. This indicates the potential of these nano-carriers for targeted-delivery. In addition, healthy cell viability was 69% for 10 μM free doxorubicin whereas for the same content of drug in DOX-loaded nanoparticles healthy cell viability increased to 80%
  6. Keywords:
  7. Gold nanoparticles ; In vitro ; Thiolated-polyethylene glycol ; A549 cells ; Cancer chemotherapeutics ; Cancerous cells ; Cell viability ; Controlled release ; Cut-off ; Functionalized ; Dialysis ; Dialysis membranes ; Polyethylene glycols ; Polyethylene oxides ; Thermoplastics ; Cells ; Cell surface receptor ; Folate binding protein ; Drug delivery system ; Drug effect ; Metabolism ; Methodology ; Tumor cell line ; Antineoplastic Agents ; Carrier Proteins ; Cell Line, Tumor ; Cell Survival ; Doxorubicin ; Drug Carriers ; Folic Acid ; Gold ; Humans ; Nanoparticles ; Receptors, Cell Surface
  8. Source: Biotechnology Letters ; Volume 32, Issue 5 , May , 2010 , Pages 649-654 ; 01415492 (ISSN)
  9. URL: http://link.springer.com/article/10.1007%2Fs10529-010-0208-x