Loading...

Liposome Nanoparticles Synthesis in order to Study the Antibiotic Drug Delivery in Simulated Environment of Body

Bahari, Mohsen | 2018

835 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50420 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Alemzadeh, Iran
  7. Abstract:
  8. In recent years, much attention has been paid to the preparation of nanoparticles as carriers for drug delivery. Nanoparticle carriers, by changing the pharmacokinetic properties of the drug, improve the function of the drug and reduce its side effects. In the manufacture of nanoparticles, various materials such as polymers, metal particles, lipids, etc. are used to transfer the drugs, which can produce a different shape and size of the particles depending on their production method. The purpose of this project is the synthesis of liposomal nanoparticles (as nano-carriers) to load an antibiotic (Eucalyptus oil) and study the release of the encapsulated drug in the simulated environment of body (PBS). Phosphatidylcholine is a phospholipid that is commonly used in the synthesis of liposomes. For this purpose, phosphatidylcholine was first extracted from soy lecithin. This operation was carried out using four different separation techniques such as solvent extraction, precipitation, static adsorption and dynamic absorption. In order to investigate the effect of solvent type, phosphatidylcholine extraction from soybean lecithin was performed with different solvents. Then the effect of the ratio of lecithin to solvent was also tested; it was observed that the ratio of 1: 7 provides the highest percentage of phosphatidylcholine purity. The precipitation test also used magnesium sulfate solution in methanol and then the effect of different concentrations of magnesium sulfate and the number of iteration of precipitation were investigated. For adsorption experiments, silica gel was used. Finally, the percentage of phosphatidylcholine extracted by HPLC test was detected 69% In the next part, the synthesis of liposomal nanoparticles and drug encapsulation in these nanoparticles were discussed. The liposomal nanoparticles were synthesized by sonication, and then the size of obtained nanoparticles before and after loading of the drug was measured. After analyzing, the size of the nanoparticles before and after drug loading was 184.608 nm and 196.69 nm, respectively. FT-IR analysis was also performed to verify the correctness of drug loading in nanoparticles. Also, in order to investigate the release of the drug and also the study of continuous drug release, the comparison of the number of formed colonies in the non-coated and the encapsulated drug in every 24 hours was investigated. Observations indicate that the effect of the encapsulated drug remains even after 8 days, while the non-coated drug did not show such an effect
  9. Keywords:
  10. Liposoms ; Nanoparticles ; Antibiotic ; Drug Delivery ; Staphylococcus Aureus ; Phosphatidylcholine ; Lecithin ; Eucalyptus Oil

 Digital Object List

 Bookmark

No TOC