Preparation and Characterization of Hydroxyapatite Nanostructures Using Natural Resources for Bone Scaffold Applications

Gheysari, Hengameh | 2017

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  1. Type of Document: M.Sc. Thesis
  2. Language: English
  3. Document No: 50401 (56)
  4. University: Sharif University of Technology, International Campus, Kish Island
  5. Department: Science and Engineering
  6. Advisor(s): Simchi, Abdolreza; Askari, Masoud
  7. Abstract:
  8. This investigation presents synthesis and characterization of pure and monophasic hydroxyapatite (Ca10(PO4)6(OH)2; HA) Nanostructures prepared by coral and oyster shell powders heated at 800 oC for 8 h as precursor via precipitation method. The morphology of HA nanostructures was controlled in the presence of various surfactants such as SDS, CTAB and PVP. The HA Nanorods synthesized by SDS were applied to fabricate bone scaffolds. Particle sizes of the HA Nanoparticles were about 20-30 nm. Pours three-dimensional HA/Ge/CMC scaffolds cross-linked by citric and oxalic acids were synthesized. In order to increase the pore size of the scaffolds, NaCl with medium (180-250 µm) and large (420-500 µm) particle sizes as well as NaHCO3 as salt porogens were used. According to SEM images, it was found that the pore size increases with the increasing sizes of the NaCl particles and the pore size distribution decreases. Therefore, NaCl with medium particle sizes was found to be suitable to fabricate uniform pours scaffolds with pore sizes in the range of 200-300 µm. The results of MTT assay showed that citric acid is more biocompatible and safe rather than oxalic acid used as cross-linker of gelatin and CMC networks
  9. Keywords:
  10. Hydroxyapatite ; Nanostructure ; Scaffold ; Crosslinking ; Bone Scaffold

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