Loading...

Fabrication of Fibrin Sheets for Tissue Regeneration

Hajian Foroushani, Zahra | 2018

753 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50944 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Abdekhodaie, Mohammad Jafar
  7. Abstract:
  8. The cornea is a transparent, avascular, multilayer, connective tissue. When the erosion process of these cells occur, where natural and quick restoration is expected, corneal disorders begin. Regeneration of corneal tissue, using a biocompatible method is a great point of interest. Nowadays, scaffold based on amniotic membrane is used as a basement membrane in the regeneration of corneal tissue. Lots of researches focused on using auto graft scaffold to decrease the inflammation and infection of an allografts and synthetic scaffolds, while overcoming amniotic membrane’s weak mechanical properties. The aim of this survey is to construct an appropriate scaffold from patient’s own blood to decrease the inflammation and infection compared to other clinical scaffolds; increase the mechanical properties to gain feasible suture with corneal tissue. It is worth mentioning that scaffold’s proper attachment to cornea resulted in less stiches and inflammation.In this study, fibrinogen and thrombin as scaffold’s main material was extracted from human blood in high concentrations. Then, fibrinogen and thrombin’s concentration was varied to enhance the young module and tensile strength of fibrin gel up to 72 and 121 KPa, respectively. Furthermore, for scaffold’s better simulation to corneal fibrous tissue, while attaining higher mechanical properties of fibrin gel, a hybrid scaffold, combining fibrin gel and Gelatin-GAG electro spinning sheets, was constructed.Results demonstrated from the hybrid scaffold, showed increased degradation time equal to 7 days which is much longer compared to fibrin gel with 38 hours of degradation time. Young module and tensile strength of fibrin gel was enhanced to 0.662 and 0.886 MPa, respectively. Young module has reached cornea’s criteria. The presence of chondroitin-sulfate in fibrin gel as a glycosaminoglycan had positive effect of growth and attachment of cells to the scaffold, while improving the simulation of extra cellular matrix of corneal tissue
  9. Keywords:
  10. Tissue Engineering ; Cornea ; Fibrinogen ; Thrombin ; Fibrin Gel ; Glycosaminoglycans ; Corneal Tissue Regeneraction

 Digital Object List

 Bookmark

No TOC