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Bioactive hybrid metal-organic framework (MOF)-based nanosensors for optical detection of recombinant SARS-CoV-2 spike antigen

Rabiee, N ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1016/j.scitotenv.2022.153902
  3. Publisher: Elsevier B.V , 2022
  4. Abstract:
  5. Fast, efficient, and accurate detection of SARS-CoV-2 spike antigen is pivotal to control the spread and reduce the mortality of COVID-19. Nevertheless, the sensitivity of available nanobiosensors to detect recombinant SARS-CoV-2 spike antigen seems insufficient. As a proof-of-concept, MOF-5/CoNi2S4 is developed as a low-cost, safe, and bioactive hybrid nanostructure via the one-pot high-gravity protocol. Then, the porphyrin, H2TMP, was attached to the surface of the synthesized nanomaterial to increase the porosity for efficient detection of recombinant SARS-CoV-2 spike antigen. AFM results approved roughness in different ranges, including 0.54 to 0.74 μm and 0.78 to ≈0.80 μm, showing good physical interactions with the recombinant SARS-CoV-2 spike antigen. MTT assay was performed and compared to the conventional synthesis methods, including hydrothermal, solvothermal, and microwave-assisted methods. The synthesized nanodevices demonstrated above 88% relative cell viability after 24 h and even 48 h of treatment. Besides, the ability of the synthesized nanomaterials to detect the recombinant SARS-CoV-2 spike antigen was investigated, with a detection limit of 5 nM. The in-situ synthesized nanoplatforms exhibited low cytotoxicity, high biocompatibility, and appropriate tunability. The fabricated nanosystems seem promising for future surveys as potential platforms to be integrated into biosensors. © 2022 Elsevier B.V
  6. Keywords:
  7. Biosensor ; MOF-5 ; Antigens ; Biocompatibility ; Biosensors ; Diseases ; Hydrothermal synthesis ; Nanosensors ; Nanostructured materials ; Nanosystems ; Organometallics ; Bioactives ; High-gravity ; High-gravity technique ; Metal-organic framework-5 ; Metalorganic frameworks (MOFs) ; Nanobiosensor ; Optical detection ; Recombinant SARS-CoV-2 spike antigen ; Synthesised ; SARS ; Cobalt ; Metal organic framework ; Nickel nanoparticle ; Porphyrin ; Coronavirus spike glycoprotein ; Spike protein, SARS-CoV-2 ; Antigen ; COVID-19 ; Metal ; Nanomaterial ; Antigen detection ; Biological activity ; Cell viability ; Chemical interaction ; Controlled study ; Coronavirus disease 2019 ; Cost effectiveness analysis ; Cytotoxicity ; HEK293 cell line ; HeLa cell line ; Hep-G2 cell line ; Human ; Human cell ; Limit of detection ; MCF-7 cell line ; MTT assay ; Nanofabrication ; Nonhuman ; PC12 cell line (pheochromocytoma) ; Porosity ; Severe acute respiratory syndrome coronavirus 2 ; Synthesis ; Chemistry ; Genetic procedures ; Procedures ; Biosensing Techniques ; Humans ; Metal-Organic Frameworks ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus
  8. Source: Science of the Total Environment ; Volume 825 , 2022 ; 00489697 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0048969722009949