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Reduced polydopamine coated graphene for delivery of Hset1 antisense as A photothermal and gene therapy of breast cancer

Babavalian, A ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1016/j.jddst.2022.103462
  3. Publisher: Editions de Sante , 2022
  4. Abstract:
  5. Breast cancer is the most prevalent type of cancer in women; hence, many researches have been focused on developing effective treatment protocols. In this study, a novel nanocarrier was fabricated for gene and photothermal combination cancer therapy by conjugating histone methyltransferase complex subunit SET1 (hSET1) on reduced polydopamine coated graphene oxide nanosheets (rGO-PDA). The rGO-PDA nanocarriers provide higher near-infrared absorption and further integrating with hSET1 antisense as an anticancer gene that down-regulates the amount of hSET1 overexpressed and suppresses the proliferation of cancer cells. The nanoplatform was prepared by polymerizing of dopamine, a mussel adhesive protein, on graphene oxide nanosheets in alkaline media, followed by conjugation of hSET1 antisense on rGO-PDA nanocarriers which was examined by gel retardation assay. After Laser irradiation, the amount of hSET1 was estimated in overexpressed cells treated with this formulation by real-time reverse transcription-polymerase chain reaction (RT-PCR). The size and zeta potential of prepared nanocarriers were 140 nm and −29.4 mv respectively. In vitro cellular tests confirmed that the prepared nanoplatform was biocompatible with no cell toxicity. The nanocarrier as a photothermal agent generating hyperthermia, led to a better release of hSET1 antisense under NIR (near-infrared) and more apoptosis of cancer cells and RT-PCR implied a considerable decrease of hSET1 expression. Outstanding photothermal conversion and suitable potential of rGO-PDA for interaction with oligonucleotides is expected as a suitable system for gene delivery and photoresponsive therapy of solid tumors simultaneously. © 2022 Elsevier B.V
  6. Keywords:
  7. Gene therapy ; Graphene oxide ; hSET1 antisense ; Photothermal therapy ; Polydopamine ; Dopamine ; Histone methyltransferase ; Histone methyltransferase complex subunit SET1 ; Mussel adhesive protein ; Nanocarrier ; Nanosheet ; Oligonucleotide ; Unclassified drug ; Antitumor gene ; Apoptosis ; Biocompatibility ; Breast cancer ; Cancer cell ; Cancer growth ; Cell proliferation ; Coating (procedure) ; Controlled study ; Cytotoxicity ; Gel mobility shift assay ; Gene delivery system ; Gene overexpression ; Gene silencing ; Human ; Human cell ; Hyperthermia ; In vitro study ; Infrared radiation ; Particle size ; Real time reverse transcription polymerase chain reaction ; Zeta potential
  8. Source: Journal of Drug Delivery Science and Technology ; Volume 73 , 2022 ; 17732247 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S1773224722003720