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Development of an in-situ forming collagen-based hydrogel as a regenerative bioadhesive for corneal perforations

Kabir, H ; Sharif University of Technology | 2024

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijbiomac.2024.134761
  3. Publisher: 2024
  4. Abstract:
  5. Corneal injuries play a significant role in global visual impairment, underscoring the demand for innovative biomaterials with specific attributes such as adhesion, cohesion, and regenerative potential. In this study, we have developed a biocompatible bioadhesive for corneal reconstruction. Derived from Collagen type I, naturally present in human corneal stromal tissue, the bioadhesive was cross-linked with modified polyethylene glycol diacrylate (PEGDA-DOPA), rendering it curable through visible light exposure and exhibiting superior adhesion to biological tissues even in wet conditions. The physicochemical characteristics of the proposed bioadhesive were customized by manipulating the concentration of its precursor polymers and adjusting the duration of photocrosslinking. To identify the optimal sample with maximum adhesion, mechanical strength, and biocompatibility, characterization tests were conducted. The optimal specimen, consisting of 30 % (w/v) PEGDA-DOPA and cured with visible light for 5 min, exhibited commendable adhesive strength of 783.6 kPa and shear strength of 53.7 kPa, surpassing that of commercialized eye adhesives.Additionally, biocompatibility test results indicated a notably high survival rate (>100 %) of keratocytes seeded on the hydrogel adhesive after 7 days of incubation. Consequently, this designed bioadhesive, characterized by high adhesion strength, robust mechanical strength, and excellent biocompatibility, is anticipated to enhance the spontaneous repair process of damaged corneal stromal tissue. © 2024
  6. Keywords:
  7. Biocompatible materials ; Polyethylene glycols ; Laser beams ; DOPA ; Dopamine ; In situ forming collagen ; Sealant ; Unclassified drug ; Biomaterial ; Collagen ; Hydrogel ; Macrogol derivative ; Tissue adhesive ; Bio adhesives ; Collagen type I ; Corneal injury ; Corneal perforation ; In-situ forming ; In-situ forming hydrogel ; Property ; Regenerative property ; Visible light ; Visual impairment ; Adhesion ; Air pressure ; Bioadhesive ; Biocompatibility ; Biodegradability ; Burst pressure measurement ; Cadaver ; Cell growth ; Cell isolation ; Cell migration ; Cell proliferation ; Cell survival ; Cell viability assay ; Controlled study ; Cornea cell ; Cornea perforation ; Cornea stroma ; Corneal curvature ; Cryopreservation ; Cytotoxicity ; Denaturation ; Drug development ; Enzymatic degradation ; Fourier transform infrared spectroscopy ; Gel permeation chromatography ; Human cell ; Human corneal stromal tissue ; Intraocular pressure ; Lap shear test ; Live/dead assay ; Mechanical test ; MTT assay ; Nuclear magnetic resonance spectroscopy ; Physical examination ; Physical polymerization ; Polyacrylamide gel electrophoresis ; Polymerization ; Pressure ; Primary cell isolation ; Proton nuclear magnetic resonance ; Scanning electron microscopy ; Shear strength ; Shear stress ; Survival rate ; Swelling ratio ; Thermostability ; Two dimensional cell culture ; Chemistry ; Drug therapy ; Materials testing ; Tissue
  8. Source: International Journal of Biological Macromolecules ; Volume 278 , 2024 ; 01418130 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0141813024055661