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Enzymatic synthesis of amoxicillin with immobilized penicillin G acylase

Alemzadeh, I ; Sharif University of Technology | 2010

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  1. Type of Document: Article
  2. Publisher: 2010
  3. Abstract:
  4. The synthesis of amoxicillin with immobilized penicillin G acylase (PGA) in aqueous medium was investigated. The parameters studied were: time course of amoxicillin production, concentration of substrates: hydroxyphenylglycine methyl ester (HPGM) and 6-aminopeicillanic acid (6-APA) and the. effect of enzyme (PGA) content and pH, under variable and constant conditions and temperature variations. In the study of two substrate concentration on amoxicillin production, impressive results were obtained for a 1/3 ratio of 6-amino penicillanic acid (6-APA) and hydroxyl-phenylglycine methyl ester (HPGM). The. synthesis of amoxicillin was preferable at constant pH rather than a variable one. Other optimal conditions obtained were: enzyme concentration: 5 g/L with 100U, process time: 480 min and temperature: 35° C. The yield for amoxicillin synthesis under prescribed conditions showed up to 50%
  5. Keywords:
  6. Enzyme content ; Amoxicillin ; Aqueous medium ; Constant conditions ; Enzymatic synthesis ; Enzyme concentrations ; Methyl esters ; Optimal conditions ; Penicillin G acylase ; Phenylglycine methyl esters ; Process time ; Substrate concentrations ; Temperature variation ; Time course ; Carboxylic acids ; Enzymes ; Esterification ; Esters ; pH effects ; Substrates ; Drug products ; Antibiotics ; Aqueous solution ; Biochemical composition ; Enzyme activity ; PH ; Temperature effect
  7. Source: Scientia Iranica ; Volume 17, Issue 1 C , 2010 , Pages 106-113 ; 10263098 (ISSN)
  8. URL: http://www.sid.ir/en/VEWSSID/J_pdf/95520101C02.pdf