Loading...

Turnip (brassica rapa) peroxidase: Purification and characterization

Motamed, S ; Sharif University of Technology | 2009

996 Viewed
  1. Type of Document: Article
  2. DOI: 10.1021/ie801997e
  3. Publisher: ACS , 2009
  4. Abstract:
  5. Partial purification of plant peroxidase from turnip (Brassica rapa) was optimized. Aqueous two phase system and precipitation by ammonium sulfate (as two parallel purification methods) were used. Polyethylene glycol/ammonium sulfate/NaCl (25:7:3%, w/v) system followed by gel-filtration chromatography gave a purification factor of about 36 fold. On the other hand, ammonium sulfate precipitation (60-80% deg) followed by gel filtration gave only about 13 purification fold. Hence, the aqueous two-phase system was more efficient and useful method as a primary purification step since it was less laborious, less time-consuming, and led to more purification factor. The partially purified turnip enzyme was stable at a pH range 2.6-6.0 and had an optimum pH at 4.0. The enzyme was stable up to 55 °C with an optimum activity at 35 °C
  6. Keywords:
  7. Ammonium Sulfate ; Ammonium sulfate precipitation ; Aqueous two phase system ; Brassica rapa ; Gel filtration ; Gel-filtration chromatography ; Optimum activity ; Optimum pH ; Partial purification ; pH range ; Plant peroxidase ; Purification factors ; Purification method ; Ammonium compounds ; Chromatographic analysis ; Enzymes ; Gels ; Plants (botany) ; Polyethylene glycols ; Polyethylene oxides ; Purification
  8. Source: Industrial and Engineering Chemistry Research ; Volume 48, Issue 23 , Volume 48, Issue 23 , 2009 , Pages 10614-10618 ; 08885885 (ISSN)
  9. URL: http://pubs.acs.org/doi/abs/10.1021/ie801997e%40proofing