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Physical, morphological, and biological studies on PLA/nHA composite nanofibrous webs containing equisetum arvense herbal extract for bone tissue engineering

Khakestani, M ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1002/app.45343
  3. Abstract:
  4. A series of herbal extract incorporated into poly(lactic acid) (PLA) composite nanofibrous scaffolds were successfully prepared by using electrospinning technique. Equisetum arvense extract (EE) and nanohydroxyapatite (nHA) in different quantities were loaded into PLA solution to fabricate composite nanofibrous webs under various electrospinning conditions. Uniform nanofibers were obtained with an average diameter of 157 ± 47 nm in the case of those containing the herbal extract. Characterization of the webs was carried out by means of Fourier transform infrared (FTIR) spectroscopy, field emission-scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), and differential scanning calorimetry (DSC) techniques. Mechanical properties, porosity, and contact angle of the prepared webs were also determined. Releasing behavior was investigated in phosphate buffer solution (pH 7.2) medium. Moreover, cell studies and osteogenic capacity were assessed in vitro using human adipose tissue-derived mesenchymal stem cell (AT-MSC). Evaluations of cell attachment, spreading, and proliferation of AT-MSC were done by SEM observation and thiazolyl blue (MTT) assay. Osteogenic differentiation capability of AT-MSC on the nanofibrous webs was analyzed by alkaline phosphatase activity and calcium content assay. It was found that with the addition of nHA and EE to PLA nanofibrous webs, their surface hydrophobicity was reduced while the tensile strength and Young's modulus were increased satisfactorily. Regarding the samples containing EE and nHA, cellular adhesion was observed with flattened normal morphology. Osteogenic differentiation of AT-MSC on PLA/nHA/EE webs showed the highest mineralization capacity after 3 weeks which, was about 1.8 and 3 times higher than that of PLA/nHA and tissue culture polystyrene as control, respectively. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45343. © 2017 Wiley Periodicals, Inc
  5. Keywords:
  6. Biopolymers and renewable polymers ; Alkalinity ; Biocompatibility ; Biodegradable polymers ; Biomechanics ; Biopolymers ; Cell culture ; Differential scanning calorimetry ; Elastic moduli ; Electrospinning ; Energy dispersive spectroscopy ; Fourier transform infrared spectroscopy ; Functional polymers ; Medical applications ; Nanofibers ; Phosphatases ; Scanning electron microscopy ; Spinning (fibers) ; Stem cells ; Tensile strength ; Tissue ; Tissue culture ; Tissue engineering ; X ray spectroscopy ; Alkaline phosphatase activity ; Biodegradable ; Biomedical applications ; Energy dispersive X ray spectroscopy ; Field emission scanning electron microscopy ; Human adipose tissue-derived mesenchymal stem cells ; Structure property relationships ; Tissue culture polystyrenes ; Plant extracts
  7. Source: Journal of Applied Polymer Science ; Volume 134, Issue 39 , 2017 ; 00218995 (ISSN)
  8. URL: https://onlinelibrary.wiley.com/doi/full/10.1002/app.45343