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Anti-HER2 VHH targeted magnetoliposome for intelligent magnetic resonance imaging of breast cancer cells

Khaleghi, S ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1007/s12195-017-0481-z
  3. Abstract:
  4. The combination of liposomes with magnetic nanoparticles, because of their strong effect on T2 relaxation can open new ways in the innovative cancer therapy and diagnosis. In order to design an intelligent contrast agent in MRI, we chose anti-HER2 nanobody the smallest fully functional antigen-binding fragments evolved from the variable domain, the VHH, of a camel heavy chain-only antibody. These targeted magnetoliposomes bind to the HER2 antigen which is highly expressed on breast and ovarian cancer cells so reducing the side effects as well as increasing image contrast and effectiveness. Cellular iron uptake analysis and in vitro MRI of HER2 positive cells incubated with targeted nanoparticles show specific cell targeting. In vitro MRI shows even at the lowest density (200 Cells/μl), dark spots corresponding to labeled cells which were still detectable. These results suggest that this new type of nanoparticles could be effective antigen-targeted contrast agents for molecular imaging. © 2017, Biomedical Engineering Society
  5. Keywords:
  6. Magnetoliposome ; Specific cell targeting ; VHH ; Carbon nanotube ; Contrast medium ; Liposome ; Nanobody ; Nanoparticle ; Antigen binding ; Breast cancer ; Cancer cell ; Cancer therapy ; Contrast enhancement ; Controlled study ; Diagnostic imaging ; Heavy chain ; Human ; Human cell ; In vitro study ; Iron transport ; Molecular imaging ; Nuclear magnetic resonance imaging ; Ovary cancer ; Priority journal ; Reticuloendothelial system
  7. Source: Cellular and Molecular Bioengineering ; Volume 10, Issue 3 , 2017 , Pages 263-272 ; 18655025 (ISSN)
  8. URL: https://link.springer.com/article/10.1007/s12195-017-0481-z