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Fabrication of hierarchically porous silk fibroin-bioactive glass composite scaffold via indirect 3D printing: Effect of particle size on physico-mechanical properties and in vitro cellular behavior

Razaghzadeh Bidgoli, M ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1016/j.msec.2019.04.067
  3. Publisher: Elsevier Ltd , 2019
  4. Abstract:
  5. In order to regenerate bone defects, bioactive hierarchically scaffolds play a key role due to their multilevel porous structure, high surface area, enhanced nutrient transport and diffusion. In this study, novel hierarchically porous silk fibroin (SF) and silk fibroin-bioactive glass (SF-BG) composite were fabricated with controlled architecture and interconnected structure, by combining indirect three-dimensional (3D) inkjet printing and freeze-drying methods. Further, the effect of 45S5 Bioactive glass particles of different sizes (<100 nm and 6 μm) on mechanical strength and cell behavior was investigated. The results demonstrated that the hierarchical structure in this scaffold was composed of two levels of pores in the order of 500–600 μm and 10–50 μm. The prepared SF-BG composite scaffolds utilized by nano and micro particles possessed mechanical properties with a compressive strength of 0.94 and 1.2 MPa, respectively, in dry conditions. In a wet condition, the hierarchically porous scaffolds did not exhibit any fluctuation after compression load cell and were incredibly flexible, with excellent mechanical stability. The SF-BG composite scaffold with nanoparticles presented a significant 50% increase in attachment of human bone marrow stem cells in comparison with SF and SF-BG scaffold with microparticles. Moreover, SF-BG scaffolds promoted alkaline phosphatase activity as compared to SF scaffolds without BG particles on day 14. In brief, the 3D porous silk fibroin-based composites containing BG nanoparticles with excellent mechanical properties are promising scaffolds for bone tissue regeneration in high load-bearing applications. © 2019
  6. Keywords:
  7. 3D printing ; Bioactive glass ; Hierarchically porous scaffold ; Silk fibroin ; Bearings (machine parts) ; Biomechanics ; Bone ; Compressive strength ; Fabrication ; Mechanical properties ; Mechanical stability ; Nanoparticles ; Particle size ; Phosphatases ; Scaffolds (biology) ; Stem cells ; Tissue regeneration ; 3-D printing ; Alkaline phosphatase activity ; Hierarchically porous scaffolds ; Human bone marrow stem cells ; Interconnected structures ; Nano- and micro-particles ; Physicomechanical properties ; 3D printers ; Fibroin ; Bone marrow cell ; Chemistry ; Cytology ; Human ; Metabolism ; Three dimensional printing ; Tissue scaffold ; Bone Marrow Cells ; Compressive Strength ; Fibroins ; Glass ; Humans ; Porosity ; Printing, Three-Dimensional ; Tissue Scaffolds
  8. Source: Materials Science and Engineering C ; Volume 103 , 2019 ; 09284931 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0928493118334763