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Removal of bisphenol A in aqueous solution using magnetic cross-linked laccase aggregates from Trametes hirsuta

Sadeghzadeh, S ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1016/j.biortech.2020.123169
  3. Publisher: Elsevier Ltd , 2020
  4. Abstract:
  5. Enzymatic removal of Bisphenol A (BPA), acknowledged as an environmentally friendly approach, is a promising method to deal with hard degradable contaminants. However, the application of “enzymatic treatment” has been limited due to lower operational stability and practical difficulties associated with recovery and recycling. Enzyme immobilization is an innovative approach which circumvents these drawbacks. In this study, laccase from Trametes hirsuta was used for BPA removal. Amino-functionalized magnetic Fe3O4 nanoparticles were synthesized via the co-precipitation method followed by surface modification with (3-aminopropyl)trimethoxysilane (APTMS). The as-prepared nanoparticles were utilized for the immobilization of laccase with the magnetic cross-linked enzyme aggregates method (MCLEAs). Activity recovery of 27% was achieved, while no immobilized laccase was observed in the cross-linked enzyme aggregates method. The performance of immobilized laccase was measured by analyzing the degradation of BPA pollutant. The maximum removal efficiency of 87.3% was attained with an initial concentration of 60 ppm throughout 11 h. © 2020 Elsevier Ltd
  6. Keywords:
  7. Bisphenol A ; Enzyme immobilization ; Laccase ; Magnetic cross-linked laccase aggregates ; Aggregates ; Enzyme immobilization ; Iron oxides ; Magnetism ; Magnetite ; Phenols ; Precipitation (chemical) ; Synthesis (chemical) ; Bis-phenol a ; Coprecipitation method ; Cross-linked enzyme aggregates ; Enzymatic treatments ; Initial concentration ; Innovative approaches ; Laccases ; Operational stability ; Magnetic nanoparticles ; 4,4' isopropylidenediphenol ; Ferric oxide ; Fungal enzyme ; Glutaraldehyde ; Magnetite nanoparticle ; Protein aggregate ; Aqueous solution ; Biodegradation ; Concentration (composition) ; Performance assessment ; Phenol ; Technical efficiency ; Biocatalyst ; Chemical analysis ; Chemical modification ; Concentration (parameter) ; Cross linking ; Enzyme activity ; Nonhuman ; pH ; Precipitation ; Priority journal ; Surface property ; Trametes hirsuta
  8. Source: Bioresource Technology ; Volume 306 , 2020
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0960852420304405