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Optimization of Insulin Releasing from Hydrogel Encapsulated Beta Cells

Abbasi Jamaati, Parisa | 2022

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54870 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Alemzadeh, Iran; Vosoughi, Manouchehr
  7. Abstract:
  8. Beta cells are responsible for secreting insulin to maintain normoglycaemia throughout the individual’s life. Type 1 Diabetes Mellitus (T1DM) is a metabolic disorder characterized by an autoimmune response that promotes the destruction of beta-cells within the pancreatic islets, resulting in lifelong inadequate insulin secretion. Encapsulating beta cells inside a semipermeable membrane to protect encapsulated cells from direct contact with the host immune system, is a new way to treat type 1 diabetes without the need for long-term immunosuppression. . In this case, the semipermeable membrane surrounds the cells and allows oxygen, nutrients, and cell products to penetrate bilaterally while not allowing antibodies to penetrate. This enables the protection of cells from both allograft and xenograft rejection, as well as autoimmune rejection related to T1DM. In this study, injectable alginate hydrogel crosslinked with calcium ions was used to encapsulate beta cells. Disodium hydrogen phosphate salt (〖"Na" 〗_"2" 〖"HPO" 〗_"4" ) was used to delay the alginate gelation time, and the properties of this system were measured by various tests such as rheological test, compressive properties, swelling rate and pore morphology. By examining different concentrations of 〖"Na" 〗_"2" 〖"HPO" 〗_"4" as a retarding agents, it was found that the hydrogel made with a concentration of 0.3 M of this salt has suitable mechanical properties, water absorption, morphology and gelation time for tissue engineering applications. After viabilty and insulin secretion test of encapsulated cells in the selected hydrogel on days 1, 3 and 7 after encapsulation, the highest values were recorded on the seventh day with 94±2.1% viability and 158.0596±3.87μIU / ml insulin secretion. In the next step, the anti-inflammatory drug curcumin, used for reducing fibrosis formation, is shown to have little effect on cell survival and insulin secretion (93±3% viability and 153.7438±3.46μIU / ml insulin secretion on the seventh day).
  9. Keywords:
  10. Beta Cell ; Insulin ; Curcumin ; Diabetes Mellitus Type 1 ; Encapsulation ; Injectable Hydrogel ; Pancreas ; Injectable Alginate Hydrogel

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