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Comparative study of commercial media to improve GMP manufacturing of recombinant human interferon β-1a by CHO cells in perfusion bioreactor
Sedighikamal, H ; Sharif University of Technology | 2022
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- Type of Document: Article
- DOI: 10.1007/s10616-022-00554-y
- Publisher: Springer Science and Business Media B.V , 2022
- Abstract:
- Chinese hamster ovary cells are the main cellular factories for production of a wide range of recombinant proteins in biopharmaceutical industry. Recombinant human Interferon beta-1a (rh-IFN β-1a), as a cytokine is broadly used to treat multiple sclerosis. In this work, the cell line producing rh-IFN β-1a was studied to improve cell density along with the specific expression. For this reason different cell culture experiments were done using different commercial serum-free media to find the appropriate media providing higher cell density. It was shown DMEMF12, DMEM:ProCHO5, and CHO-S-SFM II led to higher cell density and shorter doubling time. Next, using these media, fed-batch, and perfusion culture with temperature shift were implemented to investigate the best condition for industrial-scale manufacturing of rh-IFN β-1a in terms of higher cell density and product expression yield. The results demonstrated that CHO-S-SFM II media and a thermally biphasic condition provide enhanced expression of rh-IFN β-1a in perfusion bioreactor. © 2022, The Author(s), under exclusive licence to Springer Nature B.V
- Keywords:
- Chinese hamster ovary cells ; Fed-batch ; Perfusion ; Rh-IFN β-1a ; Specific productivity ; Beta1a interferon ; Glucose ; Lactic acid ; Messenger RNA ; Animal cell ; Article ; Cell culture ; Cell density ; Cell growth ; CHO cell line ; Comparative study ; Concentration (parameter) ; Controlled study ; Drug manufacture ; Fed batch culture ; Good manufacturing practice ; Growth curve ; Industrial production ; Metabolite ; Nonhuman ; Protein expression ; Temperature ; Viable cell count
- Source: Cytotechnology ; Volume 74, Issue 6 , 2022 , Pages 669-680 ; 09209069 (ISSN)
- URL: https://pubmed.ncbi.nlm.nih.gov/36389287