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Biological activated carbon process for biotransformation of azo dye carmoisine by klebsiella spp

Poorasadollah, D ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1080/09593330.2021.1897167
  3. Publisher: Taylor and Francis Ltd , 2022
  4. Abstract:
  5. The feasibility of employing the biological activated carbon (BAC) process to debilitate azo dye Carmoisine by Klebsiella spp. was investigated. Plate assay revealed the capability of Klebsiella spp. for removal of Carmoisine via degradation. Kinetic parameters were measured for Carmoisine debilitation by Klebsiella spp. using the suspended anaerobic process. Two types of granular and rod-shaped activated carbon were used to form the biological beds in order to study the Carmoisine debilitation in batch processes. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) were used to indicate the colonization and biofilm formation of bacteria grown on activated carbon particles (ACPs). Thin-layer chromatography (TLC), liquid chromatography–mass spectrometry (LC–MS), high-pressure liquid chromatography (HPLC) and biosorption studies demonstrated biotransformation of Carmoisine into its constituent aromatic amines during the Carmoisine debilitation in suspended anaerobic and BAC processes. The porosity of activated carbons, inoculation size and age of biological beds were the important factors affecting the viability of bacterial cells grown on ACPs and, consequently, the rate and efficiency of the Carmoisine debilitation process determined through spectrophotometry. The reusability of biological beds was demonstrated by conducting sequential batch experiments. In conclusion, the BAC process proved to be an efficient method for anaerobic dye degradation. © 2021 Informa UK Limited, trading as Taylor & Francis Group
  6. Keywords:
  7. Azo dye ; BAC-process ; Klebsiella spp ; Activated carbon ; Amines ; Azo dyes ; Batch data processing ; High pressure liquid chromatography ; Mass spectrometry ; Reusability ; Scanning electron microscopy ; Thin layer chromatography ; Activated carbon particles ; Bacterial cells ; Biofilm formation ; Biological activated carbon ; Confocal laser scanning microscopy ; Dye degradation ; Sequential batch ; Suspended anaerobic ; Bacteria ; Aromatic amine ; Carmoisine ; Unclassified drug ; Azo compound ; Azo rubin S ; Coloring agent ; Naphthalenesulfonic acid derivative ; Bacterium ; Biofilm ; Colonization ; Inoculation ; Bacterial cell ; Bacterial colonization ; Biosorption ; Cell viability ; Controlled study ; Degradation ; High performance liquid chromatography ; Kinetic parameters ; Liquid chromatography-mass spectrometry ; Nonhuman ; Porosity ; Spectrophotometry ; Chemistry ; Klebsiella ; Metabolism ; Azo Compounds ; Biotransformation ; Charcoal ; Coloring Agents ; Naphthalenesulfonates
  8. Source: Environmental Technology (United Kingdom) ; Volume 43, Issue 18 , 2022 , Pages 2713-2729 ; 09593330 (ISSN)
  9. URL: https://www.tandfonline.com/doi/full/10.1080/09593330.2021.1897167